The object of the research is the multidisciplinary schools of the Republic of Tatarstan. The purpose of the study in the context of subject-oriented instruction is to develop and test the stages of the formation of high school students’ motivational readiness for professional identity. To achieve the goal of the research we used such methods as theoretical (comparative historical analysis), empirical (the study of the students via teacher observation, questionnaire), mathematical (processing of the obtained results by means of statistical processing of research results). The particular practical usefulness of the article is in the fact that it has developed and implemented in practice the scheme for the gradual formation of the high school students’ motivational readiness for professional identity in the context of subject-oriented instruction, which makes it possible to apply the research data by teachers of multidisciplinary lyceums, gymnasiums, specialists from institutions of additional education and a system of additional professional education.