The purpose of this article is to consider the content characteristics of the process of formation of speech abilities of students of higher educational institutions, methods of teaching speech and the possibility of their application in the educational process. The main methods of research of speech competence were theoretical and methodological analysis of the problem, comparative analysis, experiment, as well as mathematical methods of data processing that allow us to determine stable relationships and relationships, trends and patterns. In the course of the research, the coherence and complexity of students ' utterances were considered at the speech level. The analysis of the products of students 'speech activity allowed us to consider the linguistic characteristics of the text at the language, speech and content-semantic levels, and psychological, operational-dynamic, motivational, cognitive, emotional, regulatory and productive components of students' speech actions.
The aim of research was to study the distribution of mast cell populations in the lungs and spleen depending on their expression of proteases in normal conditions and different periods of simulated hypoxic hypoxia.Material and methods. Hypoxic hypoxia was modeled on 87 outbred male rats using 200 liter chambers. The animals were under experimental conditions for four months. Rats were removed from the experiment after 30, 60, 90 and 120 days. After the animals were removed from the experiment, the lungs and the spleen were removed and fixed in 10% neutral buffer solution of Labiko formalin. Lung and spleen preparations were stained with hematoxylin and eosin and according to Van Gieson. For immunohistochemical studies, a panel of monoclonal antibodies was used: Anti-Mast Cell Tryptase antibody, Anti-Mast Cell Chymase antibody.Results. When modeling hypoxia in the structures of the lungs at the terms of 30 and 60 days, the content of predominantly tryptase-positive cells increased, and by the 90th and 120th days, the content of chymase-positive cells increased. By the end of the experimental exposure, the number of tryptase-positive cells increased by 3 times, and chymase-positive – by 7,7 times compared with the control. As the duration of the experiment increased, the presence of tryptase-positive cells in the spleen increased by 3,5 times, chymase-positive cells – by 7 times in the structures of the red and white pulp.Conclusion. During the formation of chronic hypoxic hypoxia in the body of laboratory animals, there is a redistribution of mast cells expressing tryptase and chymase. The most significant increase in the number of chymase-positive mast cells was noted both in the lungs and in the spleen.
www.gynecology.su 2017 • том 11 • № 2 Данная интернет-версия статьи была скачана с сайта http://www.gynecology.su. Не предназначено для использования в коммерческих целях. Информацию о репринтах можно получить в редакции. Тел.: +7 (495) 649-54-95;эл. почта: info@irbis-1.ru. РезюмеЦель работы заключалась в изучении влияния этилметилгидроксипиридина малата на абсолютное количество эритробластических островков в условиях воздействия серосодержащих газов на разных этапах онтогенеза. Материалы и методы. Эксперимент проведен на белых беспородных крысах-самцах разного возраста, подвергавшихся воздействию токсического агента (природный серосодержащий газ Астраханского газоконденсатного месторождения) и получавших и не получавших протекторного воздействия. Результаты. Выявлено, что использование этилметилгидроксипиридина малата на фоне интоксикации газообразными серосодержащими поллютантами приводит к относительной нормализации пролиферации и созревания эритроцитов на всех изученных этапах онтогенеза, что позволяет рассматривать этот препарат как эффективное средство нейтрализации негативных эффектов воздействия токсикантов. Заключение. Данное исследование поможет выявить механизмы и способы повышения неспецифической защиты в случае хронического воздействия опасных и вредных факторов окружающей среды на этапах постнатального онтогенеза. Ключевые словаЭритропоэз, серосодержащий газ, этилметилгидроксипиридина малат, крысы, этапы онтогенеза. Статья поступила: 09.01.2017 г.; в доработанном виде: 05.05.2017 г.; принята к печати: 27.06.2017 г. Конфликт интересовАвторы заявляют об отсутствии необходимости раскрытия финансовой поддержки или конфликта интересов в отношении данной публикации.Все авторы сделали эквивалентный вклад в подготовку публикации. Для цитированияОвсянникова О.А., Карпеева Д.В., Осипенко М.Д. Влияние этилметилгидроксипиридина малата на абсолютное количество эритробластических островков в условиях воздействия серосодержащего газа на разных этапах постнатального онтогенеза. Акушерство, гинекология и репродукция. 2017; 11 (2): 23-28.Данная интернет-версия статьи была скачана с сайта http://www.gynecology.su.Не предназначено для использования в коммерческих целях.Информацию о репринтах можно получить в редакции. Тел.: +7 (495) 649-54-95; эл. почта: info@irbis-1.ru. SummaryObjective. To study the effect of the protective agent ethylmethylhydroxypyridine malate on the numbers of erythroblastic islets in rats exposed to sulfur-containing gases. Materials and methods. Natural sulfur-containing gas mixtures from the Astrakhan gas-condensate field were used to model the gas-polluted environment. Male rats of various ages simulated different stages of postnatal human ontogenesis. Results. The obtained results indicate that at all stages of ontogenesis ethylmethylhydroxypyridine malate protected erythroblastic islets from intoxication by gaseous sulfur pollutants and led to partial normalization of proliferation and maturation of erythroblasts. Conclusion. The protective action of ethylmethylhydroxypyridine malate may be...
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