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This investigation compared the effects of repeated infestations to immunization of dogs with tick salivary gland or midgut extracts on the feeding and fecundity performances of female Rhipicephalus sanguineus (Latrielle). In each immunized group, three tick-naive dogs were immunized three times with tick salivary gland or midgut extracts, and twice challenged at 21-d intervals by allowing 80 female and 40 male adult ticks to feed on each host. The repeated infestation group of three naive dogs was infested five times at 21-d intervals by the same numbers of ticks. The repeated infestation group showed a trend of reduced tick performance after the third infestation, but some of the tick performance parameters had recovered by the fifth infestation. Tick attachment was reduced by immunization with either tick salivary gland or midgut extract. Immunization with tick salivary gland extract had the greatest impact on the feeding period and engorgement weight of the female ticks. Immunization with tick midgut extract resulted in the greatest reduction of tick fecundity parameters, which included preoviposition, oviposition, and egg-incubation periods in addition to reduced egg production and egg viability. These results confirm that dogs can become resistant to R. sanguineus, and demonstrate that immunization with tick salivary gland or midgut extract has different effects on tick feeding and fecundity.

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Boophilus microplus has a major impact on cattle production, and an antitick vaccine would be a valuable tool for control of this important ectoparasite in Thailand. Previous work has shown that immunization of hosts with different tick tissues has different implications regarding tick feeding and fecundity under experimental conditions. The purpose of this study was to assess the effects of immunization of dairy cattle with B. microplus salivary gland or midgut extracts on natural infestations by this tick species. The different antigen extracts (1 mg total protein) or equivalent amounts of adjuvant alone were injected intradermally every two weeks for a total of three times before allowing cattle to graze in a tick-contaminated pasture. Animals were checked daily, and engorged female ticks collected, counted, weighed, and maintained in tick incubators to observe tick performance parameters, including engorged weight, egg mass weight, nonviable eggs, mortality, oviposition period, egg incubation period, and F1 larval weight. After six months, each group was reimmunized with the same antigen and/or adjuvant, and ticks were again collected and evaluated. Immunization of cattle with salivary gland preparations resulted in reductions in mean tick counts and in engorged female weights. Immunization with midgut antigens reduced tick oviposition and reduced egg mass weights. In addition, more ticks recovered from midgut-immunized cows produce nonviable eggs. This investigation indicates that a vaccine based on these antigen preparations could induce a lasting, protective immune response against B. microplus that would be expected to provide a safe nontoxic means of tick control.