No abstract

Lacquer stellacyanin was isolated and purified from lacquer acetone powder by continuous Sephadex column chromatographies using Sephadex C-50, DEAE A-50, and C-50 gels. The purified lacquer stellacyanin had a blue color with one major and three minor bands around 26 k Dain SDS PAGE. Trypsin-and chymotrypsin-treated lacquer stellacyanins were examined by LC/MS/MS to determine three N-glycosylation sites (N28, N60, and N102) and were further analyzed by MALDI TOF MS, indicating that the Nlinked glycans were attached to the three asparagine (Asn) sites, respectively. In addition, after trypsin digestion and PNGase A and PNGase F treatments to cleave N-linked glycans from the Asn sites, it was found that lacquer stellacyanin had a xylose containing a biantennary N-linked glycan with core fucosylation consisting of 13 sugar residues (a complex type N-linked glycan) by MALDI TOF MS analysis. This is the first report on the structure of an N-linked glycan in lacquer stellacyanin.

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We have defined the biochemical composition of the carcass meat of muskrat, including the content of the amino acids, lipids, and saturated and unsaturated fatty acids. The moisture, crude proteins, fatty acid, and ash were 74.6, 23.2, 1.2, and 0.92%, respectively. The average amount of calories was 478 kJ per 100 g meat. We separated 17 amino acids using reversed-phase high-performance liquid chromatography (RP-HPLC) analysis. The fat content was low in both male and female invasive muskrats. The aliphatic tails of the fatty acids range from 16 to 18 carbons. The saturated palmitic (C16:0) and stearic acids (C18:0), and unsaturated oleic (C18:1) and linoleic (C18:2), and linolenic acids (C18:3) were extracted. Even though some of the western provinces in Mongolia applied the carcass meat of the muskrats for treatment against kidney disease in traditional medicine, the scientific proof is still unclear.