Origanum onites (Lamiaceae) is an Eastern Mediterranean plant that is widely used in Turkish traditional medicine. This study aimed to investigate the biochemical composition, antimicrobial activity, and antioxidant potential of O. onites. In this study, the biochemical composition of the O. onites ethanol extract (OOEt) was analyzed using GC-MS. The antimicrobial activity was investigated using a disk diffusion test and determining minimum inhibitory concentrations (MIC) against 30 microorganism strains, including 28 bacteria (some multidrug-resistant) and 2 fungi. Additionally, the antioxidant activity was evaluated using the DPPH method. The main component identified was carvacrol. OOEt demonstrated antimicrobial activity against a wide range of tested microorganism strains. OOEt displayed the highest activity against E. faecium (a Gram-positive bacterium) at 100 µL with a 52 mm inhibition zone. Additionally, P. aeruginosa DSMZ 50071 and P. fluorescens P1, which are Gram-negative bacteria, were the most sensitive strains with a 24 mm inhibition zone in 100 µL of OOEt. The data obtained from A. baumannii (a multidrug-resistant strain) is particularly striking, as higher activity was observed compared to all positive controls. All tested fungal strains showed more effective results than positive controls. The antioxidant activity of OOEt was found to be stronger than that of the positive control, ascorbic acid. This study determined that O. onites has significant antimicrobial and antioxidant potential.

Karayosunlarının hastalıklara karşı etkisi olduğu çok uzun yıllardan beri bilinmektedir ve yaraların tedavi edilmesi ve enfeksiyonlar ile mücadele gibi alanlarda etkin olarak kullanılmaktadırlar. Bu çalışmada Plagiomnium elatum, Rhynchostegium megapolitanum, Tortella fasciculata ve Tortella tortuosa karayosunu örneklerinin etil alkol ekstraktları disk difüzyon yöntemi kullanılarak, 8 gram pozitif, 9 gram negatif bakteri ve 1 maya olmak üzere, toplam 18 mikroorganizma üzerinde test edilerek antimikrobiyal aktiviteleri belirlenmiştir. Disk difüzyon testi sonucunda tüm karayosunu örneklerinin birkaç mikroorganizmaya karşı antimikrobiyal aktiviteye sahip olduğu ve özellikle gram pozitif olan Staphylococcus aureus ATCC 25923 ve gram negatif olan Escherichia coli ATCC 25922 üzerinde belirgin bir antimikrobiyal aktivitesinin olduğu ortaya konmuştur.

No abstract

In this study, the biochemical, antioxidant properties, and antimicrobial activity of the epiphytic leafy liverwort Frullania dilatata (L.) Dumort were investigated. Due to the scarcity and difficulty in obtaining liverworts, research on their bioactivity is limited; thus, this study aimed to uncover the potential of F. dilatata. The antimicrobial activity was evaluated against various microorganisms, including food isolates, clinical isolates, multidrug-resistant strains, and standard strains, using the disk diffusion method and determining the minimum inhibitory concentration (MIC) values. This study represents the first antioxidant investigation on F. dilatata and an antimicrobial study using ethanol extract and the disk diffusion method. Notably, susceptibility was observed in Enterococcus faecalis ATCC 29212, Enterococcus faecium FI, Listeria monocytogenes ATCC 7644, Providencia rustigianii MDR, and Staphylococcus aureus ATCC 25923. The antioxidant capacity was assessed using the DPPH method, emphasizing the high scavenging performance. Gas chromatography-mass spectrometry (GC-MS) analysis identified the primary compounds as frullanolide (19.08%), 2,3-Dimethylanisole (15.21%), linoleic acid (11.11%), palmitic acid (9.83%), and valerenic acid (5.3%). The results demonstrated the significant antimicrobial activity of F. dilatata against the tested microorganisms and its potent antioxidant properties. These findings emphasize the potential of F. dilatata as a promising source of natural antimicrobial and antioxidant agents, underscoring the importance of further investigation into its bioactive compounds and elucidating the mechanisms of action in future studies.

Bu çalışmada; Kastamonu ili sınırları içerisinde gerçekleştirilmiş ciğerotu florası araştırmaları incelenerek, ilin ciğerotu florasının güncel takson listesinin hazırlanması amaçlanmıştır. Buna göre alanda yapılan çalışmaların incelenmesi neticesinde Kastamonu ilinde 1 boynuzotu (Anthoceratophyta) taksonu ile 29 familyaya ait 73 ciğerotu (Marchantiophyta) taksonu bulunduğu tespit edilmiştir. Scapaniaceae familyası 9 takson ile en zengin familya konumundadır.