Photodynamic therapy (PDT) is a promising treatment modality in the management of premalignant and malignant conditions. Nanoparticles were used recently as drug delivery agents and have shown increase in the efficacy of PDT. In this study we have used gold nanoparticles as a potential drug delivery agent in enhancing the photodynamic efficacy. Glutathione (GSH) capped gold nanoparticles were synthesized by immobilizing the thiol group of the GSH on to the surface of the gold nanoparticles. Rosebengal, a hydrophilic photosensitizer was conjugated to the amine group of the bounded GSH. These synthesized rosebengal conjugated gold nanoparticles were characterized using FT-IR spectroscopy for confirmation of the complex formation. Further, studies on the absorption, emission and lifetime of the nanocomplexes-show significant changes due to the formation of the complex. The PDT efficacy of these nanocomplexes and sensitizer alone were studied against Vero and HeLa cell lines. The phototoxicity results shows the nanocomplexes are more phototoxic than that of the free rosebengal where the covalent complex being the better of the nanocomplex.

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