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A panel of monoclonal antibodies (MAbs) was prepared and used to develop an enzyme immunodot assay for the rapid identification and presumptive serotyping of aquatic birnaviruses. Comparison of the reaction patterns of these MAbs with representative virus isolates indicated that one MAb recognizes a serogroupreactive epitope and can therefore be used for identification of all serogroup A aquatic birnaviruses, the predominant serotype worldwide. Other MAbs exhibited more restrictive specificities, permitting the presumptive serotyping of viruses of the three recognized serotypes and the identification of some individual strains. This assay, in which MAbs are used, is more efficient in terms of time, cost, and ease of performance and provides significant advantages in specificity and standardization compared with currently used tests.

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A panel of 11 monoclonal antibodies (MAbs) was used in an immunodot assay to isolated in Norvvay from different speeies of both earrier and diseased fish. All the 81 isolates shared a serogroup A-speeifie epitopc, and 67 of them had a reaetion pattern identieal to the Sp-type strain. Of the other 14 isolates, nine resembled the Sp-type strain, two resembled both the Sp-and He-type strains, two were elosest to the Ab-type strain and one resembled the Te-type strain. Sp-rclated strains were predominant from all nine host groups, including from both Atlantic salmon, Salmo salar L., with infectious pancreatie necrosis (IPN) and healthy Atlantic salmon. The Nl strain reaeted identically to the Sp-type strain.Correspondence: Hans Peter Melby, P.O.B. 8156 Dep, N-()(I33 Oslo, Norway.