Four Holstein cows were used in a 4 × 4 Latin square design to determine the effect of feeding different processing whole cottonseed (WCS) on milk yield, milk composition and milk fatty acid profi le. Result showed that dry matter intake and milk yield were unaffected by different treatments, but milk protein concentrations were increased in cows fed processing WCS diets. Compared with control, feeding cows with different processing WCS diets reduced C14:0 and C16:0 and elevated C18:0 and C18:1 in milk, and feeding extruded delinted WCS increased C18:2 and cis9, trans11 conjugated linoleic acid in milk.
Four ruminally cannulated Holstein cows were used in a 4 × 4 Latin square design to determine the effect of feeding linted, delinted and extruded linted whole cottonseed (WCS) on nutrient digestibility and ruminal fermentation. Result showed that crude protein digestibility was lower in linted and delinted WCS treatments than control and extruded linted WCS treatment. Cows fed extruded delinted WCS had the highest digestibility of neutral detergent fi bre compared with those fed the other diets. The inclusion of processing WCS did not alter ruminal pH and ruminal total and molar proportion of volatile fatty acids, however, feeding delinted WCS reduced the ruminal ammonia nitrogen concentration and acetic:propionic acid ratio.
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