Lead being a toxic cumulative poison and an environmental pollutant, experiments were conducted at an oral chronic dose of (60 mg/kg/day) for 90 days on adult female rats (Rattus Norvegicus) and its effect on the reproductive functions in relation to the biochemical effects was studied. It was observed that the chronic dose of lead caused an elevation in the level of proteins, acid phosphatase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase in all the soft tissues studied indicating tissue damage. It also inhibited the level of acetylcholinesterase in all the tissues. Fertility tests by pairing treated females with males showed that lead-treated female showed irregular estrous cycle and the fertility rate dropped to 40% as female pups of lead-treated mothers showed loss in weight, high mortality rate, poor growth rate, and late vaginal opening. Histological studies of ovary showed atresia in all the stages of folliculogenesis sustaining the poor fertility observations. The present study revealed that lead caused great tissue damage and affected reproductive performance of female rats at a chronic dose.