Coumarin derivatives possess a wide spectrum of biological activities (1-3). Also, it is well documented that pyrazoles, pyrazolin-5-ones, 4-thiazolidinones and 1,3,4-oxadiazoles display pronounced antioxidant (4-6) and antineoplastic activity (7-10). In view of the considerable importance of the coumarins and heterocycles mentioned above, the present work is aimed at the design and synthesis of new heterocyclic compounds bearing coumarin moiety. Moreover, the study includes testing of target compounds for their cytotoxic activity against Dalton's lymphoma ascites (DLA) and Ehrlich ascites carcinoma (EAC) cells.
We report a simple, cost-effective, and label-free detection method, consisting of a platelet-derived growth factor (PDGF) binding aptamer and hydrophobic Ru(II) complex as a sensor system for PDGF. The binding of PDGF with the aptamer results in the weakening of the aptamer-Ru(II) complex, monitored by luminescence signal. A substantial enhancement in the luminescence intensity of Ru(II) complex is observed in the presence of aptamer due to the hydrophobic interaction. Upon addition of PDGF, the luminescence intensity is decreased, due to the stronger interaction between the aptamer and PDGF resulting in the displacement of Ru(II) complex to the aqueous solution. Our assay can detect a target specifically in a complex medium such as the mixture of proteins, at a concentration of 0.8 pM.
A series of substituted phenyl methyl piperazine triazolyl benzotriazoles 4a, 4b, 4c, 4d, 4e, 4f, 4g have been synthesized through the Mannich reaction of substituted phenyl triazolyl benzotriazoles 3a, 3b, 3c, 3d, 3e, 3f, 3g. The substituted phenyl triazolyl benzotriazoles were prepared from benzotriazolyl acetohydrazide, where the cyclization was facilitated through ammonium acetate and aryl aldehydes. The IR, 1H NMR, mass spectral data and elemental analysis were performed to assign the structure. All the newly synthesized compounds were screened for their antimicrobial and antioxidant activity.
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