Conclusion:Influence of ethnicity on fetal nasal bone length was not significant. Our preliminary results showed for the Brazilian population was not necessary the first-trimester screening adjustment for ethnicity. Objective: To assess the relationship between amniotic fluid sample volume, culture time and culture failure in prenatal diagnosis. Method: We retrospectively evaluated the results of 225 cases of genetic amniocentesis performed between 16 and 20 weeks of gestation. Cases were separated into four groups according to the amount of amniotic fluid obtained: Group A (n = 12, 1 to 5 mL); Group B (n = 23, 6 to 10 mL); Group C (n = 10, 11 to 15 mL); Group D, (n = 180, 16-20 mL). Results: Culture time was significantly longer in Group A than in the other groups. (median 19 days vs. 12.5, 10, 11 days). Even if differences in culture time within Groups B to D were not significant, there was a trend towards an increase in culture time with decreasing amniotic fluid volumes. Moreover, risk of culture failure was also increased with smaller volumes. There were no culture failures in Groups B, C and D but two culture failures in Group A. Conclusion: When necessary, small amniotic fluid volumes (at least 10 mL) may be used for fetal karyotyping with confidence after information of the family. Small volumes of amniotic fluid increase culture time and rate of culture failure. P01.33 P01.34 Evaluation of complications after the genetic amniocentesis 2nd Department of Obstetrics and Gynecology, Warsaw, PolandObjective: To evaluate complications after genetic amniocentesis. Methods: Retrospective analysis of 1153 women who underwent genetic amniocentesis from 1999 to 2005, which was done by ultrasound-guided insertion of a 22-gauge needle. The mean maternal age was 37 ± 4 years. 1148 (99%) women were in singleton pregnancy, 4 (0.03%) in twin pregnancy and one (0.08%) in triplet pregnancy. Results: Amniocentesis was performed between 12 and 32 weeks' gestation (mean 14 ± 1 weeks). The most common indication for amniocentesis was maternal age over 35 year -988 (86%). 17 (1.4%) patients had pregnancy loss after amniocentesis: 7 (0.6%) women had missed abortion and 10 (0.8%) had amniotic fluid leakage. 1 (0.1%) had pain in hypogastrium after the procedure, but she delivered at term. In 8 (0.7%) cases the cell culture of amniotic fluid was unsuccessful and procedure was repeated. Conclusions:The pregnancy loss rate in women who underwent genetic amniocentesis was 1.4%. P01.35Ultrasound screening for chromosomal abnormalities by fetal nuchal translucency measurement between 11-14 weeks of gestation Federal University of Espirito Santo, UFES, University Hospital HUCAM, Vitoria Medfetus, BrazilObjective: To assess the feasibility of nuchal translucency [NT] measurement as a screening tool for chromosomal anomalies between 11-14 weeks of gestation. Methods: A longitudinal prospective follow up study was carried out at a tertiary referral center including 3263 singleton pregnancies between 11 and 14 weeks of gestation. Two experi...

Perylene bisimide (PBI) dyes are among the most extensively studied functional π‐building blocks and have outstanding optical and electronic properties. Most PBI assemblies that have been reported so far are based on one‐dimensional stacks formed mainly by directional π–π interactions between quadrupolar π‐systems of PBIs. More complex and elaborate supramolecular assemblies of PBIs can be constructed by using highly directional and specific intermolecular glues, such as complementary multiple hydrogen bonding interactions, and the resulting assemblies are expected to show unique functional properties. In this Focus Review, we will discuss highly‐organized assemblies of PBI dyes, the self‐assembled architectures of which are rationally controlled by directional, complementary multiple hydrogen‐bonding interactions. An overview of the construction principles of such systems is included, but special attention is focused on the significant progress achieved after the seminal review about supramolecular architectures of PBI dyes by Würthner in 2004.

On the ''4 optimal chamber view'', we propose to check 4 key points: pulmonary inferior veins attaching the heart to lungs, in a strict axial plane; 4 chambers, mobile, balanced and concordant with a normal offset of the AV valves on the crux of the heart. We also describe the difficulties and the possible traps of the 4 chamber view. We justify each key-point by the pathology it helps diagnose. P294Evaluation of fetal heart anatomy at the end of the first trimester and at the beginning of the mid-trimester J. H. Dangel, A. Hamela-Olkowska, T. Lipiñski, R. Makowski, P. Milewczyk and K. M. Wiech PolandObjective: To evaluate fetal heart anatomy in the late first and in the early second trimester when scan was performed by cardiologist and obstetrician. Methods: The study included 88 pregnant women between 11 and 19 weeks' gestation. 87 women were in single pregnancy and one in quadrupled pregnancy. The indications for fetal echo were: high risk of chromosomal abnormalities due to maternal age -78, family history of congenital heart defects -6, increased nuchal translucency -2, quadrupled pregnancy -1 case. Exams were performed using transabdominal or transvaginal probes. Results: The proportion of cases successfully visualized to all cases in particular week of pregnancy was following: 11 week -0/1 (0%), 12 -1/1 (100%), 13 week -2/4 (50%), 14 -22/28 (78%), 15 -9/12 (75%), 16 -24/28 (85%), 17 -6/7 (85%), 18 -8/8 (100%), 19 -2/2 (100%). In 11 weeks' gestation two ventricles and two atrias could be imaged. Anatomy was seen from 12 weeks' gestation with transvaginal and from 13 weeks' gestation with transabdominal transducers. One ventricular septal defect and two tricuspid regurgitations were detected. The interventricular septum was the most difficult structure to visualize in all cases. Great vessels were not well visualized when exams were performed by obstetricians and read by cardiologist. The grey scale alone was not sufficient for accurate examination of the heart and it was necessary to use colour Doppler to confirm normal forward flow to both ventricles and to identify outflow tracts. Conclusions: 1. Successful visualization of the heart in early pregnancy is possible from 12 weeks' gestation, but its quality depends on presentation of the fetus, weight of mother, ultrasound equipment and experience of the examiner. 2. It is essential to use colour Doppler to evaluate fetal heart anatomy in early pregnancy. 3. The hemodynamics of fetal circulation in the first trimester needs further investigations. Results: The most common CHD was atrioventricular septal defects (35 cases) and hypoplastic left heart syndrome (30 cases). 91 (46%) fetuses had isolated CHD, 64 -other organs anomalies, 45 -abnormal karyotype: trisomy 18 in 23, trisomy 21 in 9, trisomy 13 in 5, Turner syndrome in 2, triploidy in 2, unbalanced translocation in 3, deletion of chromosome 22 in one case. Pregnancy was terminated in 17 (8.6%) cases. 16 fetuses died in utero (all with intrauterine heart failure). 125 neonates were live-born. Conclusio...