In current study, the phytochemical, antioxidant and cytotoxic effects of methanol extracts of Vitex negundo, Lantana camara, Bauhinia variegata and Bauhinia racemosa were reported. Methanol extract of medicinal plants (V. negundo, L. camara, B. variegata and B. racemosa) were extracted using different extraction methods and then tested for their antioxidant activity by 2,2-diphenylpicrylhydrazyl, superoxide scavenging method, chelation of metal ions and cytotoxic potency using MTT assay on human cancer cell lines. In different extraction methods, highest yield was obtained with hot extraction method. Total phenolic and flavonoid contents were found higher in methanol extract of V. negundo having 173.3±1.54 mg gallic acid equivalent/g DW and 24.41±1.5 mg quercetin equivalent/g DW contents, respectively. Leaves of V. negundo have maximum IC 50 value of 73±2.76 µg/ml and 350±1.23 µg/ml in 2,2-diphenylpicrylhydrazyl and iron chelating activity compared to all other methanol extracts. B. racemosa leaves have higher superoxide scavenging effect with IC 50 value of 223±1.23 µg/ml. V. negundo and L. camara leaves showed pronounced cytotoxic effect against HELA and KB human cancer cell lines with LD 50 value of 222±3.35 and 188.69±1.4 µg/ml, respectively. The present study shows that V. negundo and L. camara may be a probable source of natural antioxidant and as an anticancer drugs.