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Chlamydia as primary pathogens in pneumonia in cattle (KIUCHI and INABA, 5) and sheep (MCKERCHER, 9) are well established. There has, however, been a paucity of reports on animal chlamydiosis, especially chlamydial pneumonia in India. Based on adequate sero-evidence in apparently healthy cattle (53 O/o), sheep (43.6 O/o) and buffaloes (44 O/o) (DHINGRA, 3), a pilot study on the isolation of chlamydia from pneumonic lungs of buffaloes, cattle and sheep was undertaken. Material and MethodsPneumonic lung pieces from buffaloes (9) and sheep (19) were obtained from abattoirs and from cattle (3) at necropsy. All samples were kept in chilled transport medium containing antibiotics (WHO,9) and transported to the laboratory immediately. A 20 O/o inoculum was prepared in sucrose potassium glutamate (SPG) medium (BOVARNICK et al., 1) and tested for sterility. Samples found contaminated with non-pathogenic organisms, as revealed by cultural tests, were inoculated as such into healthy guinea pigs. The possibility of latent chlamydial infection in experimental guinea pigs was ruled out by testing paired sera for chlamydial group antibodies. Two guinea pigs were inoculated intra-peritoneally with 2 ml. of inoculum. One guinea pig was inoculated similarly with SPG medium as control. Rectal temperature was recorded. After an observation period of 2-3 weeks, inoculated animals were sacrificed and the gross pathology of the viscera was examined. From spleens showing splenomegaly two impression smears were prepared. One smear was stained with Giemsa stain and if cytoplasmic elementary body inclusions were observed, the spleen was processed in embryonating eggs for demonstration of elementary bodies (EB) in Gimenez stained yolk sac (YS) smears. The other smear was fixed in chilled acetone and stained with fluorescein isothiocyanate conjugated antiserum (prepared against the Bour strain of C. trachomatis) to determine the presence of group antigen. Results and DiscussionNone of the 31 pneumonic lungs processed for isolation of chlamydia could be made absolutely free from contaminants by the antibiotics (Strepto-U. S.

Summary A case of oral dermatophilosis in a buffalo calf is reported. The lesions were present on tongue, gums and cheeks. Organisms indistinguishable from Dermatophilus congolensis were demonstrated in the lesions and could be isolated. The isolate of Dermatophilus congolensis when used for experimental reproduction of the disease in buffalo calves produced mild and limiting disease with multiplication of the organisms in the lesion. Zusammenfassung Dermatophilose (Streptothrichose) bei einem Büffelkalb (Bubalus bubalis) Es wird von einem Fall oraler Dermatophilose bei einem Büffelkalb berichtet. Die Läsionen befanden sich auf der Zunge, den Gaumen und Backen. Der Erreger, der sich als typisch für Dermatophilus congolensis erwies, konnte in den Läsionen nachgewiesen und isoliert werden. Das Isolat von Dermatophilus congolensis, das für die experimentelle Reproduktion der Krankheit bei Büffelkälbern verwendet worden war, verursachte eine leichte und begrenzte Erkrankung mit Vermehrung der Erreger in den Läsionen. Résumé On rapporte un cas de dermatophilose buccale chez un veau de buffle. Les lésions se situaient sur la langue, le palais et les joues. L'agent, qui a montré les caractires de Dermatophilus congolensis, a pu être mis en évidence dans les léions et isolé. La souche de Dermatophilus congolensis utilisée pour la reproduction expérimentale de la maladie chez des veaux de buffle provoqua une légère affection limitée avec multiplication du germe dans les lésions. Resumen Dermatofilosis (estreptotricosis) en un ternero de búfala (Bubalus bubalis) Se informa sobre un caso de dermatofilosis oral en un ternero de búfala. Las lesiones se encontraban en la lengua, el paladar y carrillos. El agente etiológico, que resultó ser típico para Dermatophilus congolensis, se pudo identificar en las lesiones y aislar. El Dermatophilus congolensis aislado, que se había utilizado para la reproducción experimental de la enfermedad en terneros de búfala, ocasionó una afección ligera y limitada con multiplicación de los agentes en las lesiones. Dermatophilose (Streptothricose) chez un veau de buffle (bubalus bubalis)