Gongronema latifolium is highly medicinal in nature. The fundamental ingredients used for medicinal purposes are stored in the various parts of the plant such as the fruits, seeds, leaves, root and stem. This present study is aimed to evaluate the hepatotoxicity effect of methanolic leaf extract of Gongronema latifolium on albino rats. This study was divided into five groups normal control groups: received commercial rat feed and water, group 2: received 1000 mg/kg b.w. of leaf extract of Gongronema latifolium, group 3: received 500 mg/kg b.w of leaf extract of G. latifolium, group 4; received 250 mg/kg of leaf extract of Gongronema latifolium, and group 5: received 125mg/kg of leaf extract of Gongronema latifolium. The result shows a significant (p<0.05) increase in serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total and conjugate bilirubin when compared to the normal control not giving the extract. Administration graded dosage of 1000mg/kg and 500mg/kg body weight significantly (p<0.05) increased the liver damage marker enzymes when compared with groups that received low dosage of 250mg/kg and 125mg/kg body weight and the normal control groups. The histopathological study revealed severe portal inflammation without steatosis and moderate portal inflammation in groups that received 1000mg/kg and 500mg/kg. Therefore, these results suggested that methanol leaf extracts of Gongronema latifolium possess hepatotoxic properties and strict caution must be observed when using the plant extract as a natural remedy of any disease.

Medicinal Plants have demonstrated history of managing some ailments caused by free radicals as a result of some chemical constituents they possess. This study was aimed at assessing antioxidant and free radical scavenging properties of Tannin-rich and Flavoniod-rich fraction of Annona senegelensis and Vernonia amygdalina leaves via in vitro assays such as; reducing power, nitric oxide scavenging activity, Hydrogen peroxide scavenging activity and Lipid peroxidation scavenging activity. The results obtained indicated that both medicinal plants are antioxidant reservoir. The values for Tannin rich fraction of Annona senegelensis (TRFAS) and Tannin rich fraction of Vernonia amygdalina (TRFVA) are Reducing power absorbance TRFAS (0.077-0.187), TRFVA (0.168-0239). % Nitric oxide scavenging: TRFAS (4.46-30.40), TRFVA (5.23-42.24). % H2O2 scavenging: TRFAS (7.30-20.35), TRFVA (8.12-22.32). % Lipid Perioxidation: TRFAS (6.81-32.76), TRFVA (5.16-26.16). Also the values for Flavonoid rich fraction of Annona senegelensis (FRFAS) and Flavonoid rich fraction of Vernonia amygdalina (FRFVA) are reducing power absorbance: FRFAS (0.109-0.342), FRFVA (0.124-0.388). % Nitric oxide scavenging: FRFAS (33.55-43.57), FRFVA (21.10-47.46). % H2O2 scavenging: FRFAS (39.01-74.96), FRFVA (45.80-75.20). % Lipid Perioxidation: FRFAS (24.81-59.69), FRFVA (41.43-59.98). The Tannin-rich and Flavonoid-rich fraction of both plants exhibited good antioxidant activity on all models employed at increasing concentrations but Flavoniod-rich fraction of Vernonia amygdalina had the highest inhibitory effect than the Tannin fraction. The findings from this study validated the pharmacological potency of the two plants and their potential use in combating free radical-related diseases, which are often triggered by oxidative stress.

The body during normal metabolic function produces free radicals which are highly reactive species. Free radicals could also be introduced into the body from the environment. The oxidation induced by reactive oxygen species can result in DNA mutation, membrane protein damage and cell membrane disintegration. The present study assayed the preliminary phytochemicals, total phenolics and total flavonoids and free radicals scavenging ability of methanol extract of Jatropha tanjorensis leaf. Standard methods for determining phytochemicals, reducing power, nitric oxide scavenging, hydroxyl radical, and lipid peroxidation scavenging activity were employed.  The phytochemical screening result revealed the presences of phenols, flavonoids, saponin, alkaloids, tannins, terpernoids and steroids. The total phenolic content of methanol extract of Jatropha tanjorensis leaf measured by Folin-Ciocalteau reagent in terms of gallic acid equivalent was 11.35±0.82mgGAE/g. The flavonoid content of the plant sample calculated as Quercetin equivalent was 15.91±1.60mgQCE/g. GC-MS results revealed relevant pharmacological bioactive compounds. The antioxidative activity of the plant’s extract correlated with total phenolic content. The radical scavenging activity showed a dose dependent increase in the reducing power. The minimum NO inhibitory activity was 8.88±0.63 at 200µg/ml and the maximum activity was 32.70±2.71 at 800µg/ml. The minimum percentage H2O2 radical inhibitory activity was 8.30±0.88 at 200µg/ml and a maximum activity was 22.80±2.28 at 800µg/ml. There was also an increase scavenging effect of lipid peroxide radical in concentration dependent manner. The results of this study indicate that the leaf of Jatropha tanjorensis possess antioxidant properties and could serve as free radical inhibitor

Co-intake-related interactive-synergistic influence of artemether-lumefantrine, AL and monosodium glutamate, MSG that separately mediated oxidative stress could be significant on the kidney actively involved in xenobiotic detoxification and elimination. Thus, influence of AL on rats’ kidney histomorphology and antioxidant bio-indicators following MSG-challenge was assessed. For 7 days, thirty rats (n = 5) were respectively exposed to vehicle (distilled water), therapeutic AL (TAL), high AL (HAL), MSG, MSG plus TAL or MSG plus HAL. Significant (P<0.05) results comparison showed highest and least (P<0.05) albumin concentration (Mg/dl) in TAL-fed (3.76±0.33) and MSG-fed (1.88±0.70), rats. Total protein concentration (Mg/dl) in MSG-fed (4.04±2.04) and HAL-fed (4.76±1.92), rats lowered markedly. Highest glutathione peroxidase activity (IU/L) in TAL-fed (30.74±12.46) lowered in MSG plus HAL-fed (20.11±6.08) and MSG-fed (20.33±4.85), rats. Catalase activity (IU/L) in control was highest (4.89 ± 0.26) but least (2.58 ± 1.06) in MSG-fed rats. Zinc and Magnesium concentration (Mg/dl) was respectively highest (58.99±5.10) and least (3.48±0.31) in MSG plus HAL-fed but least (18.80±7.77) and highest (4.38±1.67) in MSG-fed, rats. Malondialdehyde concentration (µmol/ml) in MSG plus HAL-fed rats (4.04±0.67) was highest (P<0.05) and least (P<0.05) in HAL-fed rats (1.18±0.11). Differences in superoxide dismutase activity (IU/L) were, however, non-significant (P>0.05).Rats’ kidney photomicrographs (H&E × 400) revealed normal histo-architecture in control but varied degree of fibroplasias (TAL- ,HAL- and MSG plus TAL-fed) and necrosis with infiltrations (MSG plus HAL-and MSG-fed), rats. These demonstrated MSG-related adversity and significant modulation response of TAL, unlike HAL, on the rats’ kidney histology and studied antioxidant response bio-indicators.

Herbal preparations of different Morinda plant species had been used as supplements and for therapeutic purposes with less emphasis on the bioactive components. This study is aimed at investigating the in vitro antioxidant potentials of methanol fruit extracts of Morinda citrifolia (MCE) and Morinda lucida. The potent bioactive agents in MCE and MLE were extracted with 90% methanol. Both plants species did not have catalase activity but scavenged 1, 1-diphenl-2picrylhydrazyl radical (DPPH). in concentration dependent manner against percentage inhibition showing effective concentration at 50% inhibition (EC50) of 716.09 µg/ml and 910.24µg/ml for MCE and MLE compared to that of vitamin C standard with the EC50 of 54.61µg/ml. The MCE and MLE scavenged superoxide (SO) radical in concentration dependent manner with the EC50 of 72.77µg/ml and 82.43 µg/ml respectively compared to that of vitamin C standard with EC50 of 176.08µg/ml. Also, MCE and MLE scavenged nitric oxide (NO) radical in concentration dependent manner with the EC50 of 2198.32µg/ml and 3734.26µg/ml compared to that of vitamin E standard with EC50 of 1700.73µg/ml. Both MCE and MLE scavenged hydroxyl (OH) radical in concentration dependent manner with the EC50 of 134.32µg/ml and 155.07µg/ml respectively compared to that of vitamin E standard with EC50 of 54.33µg/ml. These might imply that MCE is a better antioxidant than MLE. The MLE contained more vitamin A (2,438± 0.1i.u) than MCE (1,323± 0.3i.u) while MCE vitamin C (10.80± 0.05i.u) and vitamin E (0.15± 0.2 i.u) contents was higher than that of MLE (vitamin C (7.40± 0.02i.u) and vitamin E (0.14±0.01i.u). These could also imply that MCE had more antioxidant activity and could be preferred in Nigeria folk medicine.