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A gas chromatographic/mass spectrometric analytical procedure is described to determine glycols in plasma as cyclic butyl boronate esters. The method, involving a pre-deproteinization step, required only 0.25 ml of plasma and a short time (20 min) to react with the derivatizing agent (butyl boronic acid). The gas chromatographic separation on a CP Sil 8 CB silica capillary column coupled to a mass detector assured a complete identification of the compounds. The analytical recoveries (greater than 95%) with low coefficient of variation (4-11%) assured the feasibility of the method over a concentration range from 5 to 1000 micrograms ml-1 for each glycol. The lower detection limits, namely 1-5 micrograms ml-1, confirmed the sensitivity of the method.

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Short Communications position indicated in brackets) with the respective chromatograrns ofihe pure compounds PS and PEA the discrete peaks of mpolymer CpPy products can be identified. References[I] W. Simon and il. Giacobbo, Thermische Fragrnentierung und Strukturbestirnmung organischer Verbindungen. GIT 37 (1965) 709-714.[2] R. L Levy, Chromrtugr. Rev. 8 (i966) 48.

SummaryCalcium blocker drugs are of increasing therapeutic relevance. Verapamil, nifedipine, diltiazem, and flunaririne are the most widely applied drugs of this type. New dihydropyridines (felodipine, nitrendipine) and related agents are presently undergoing intense investigation. HRGC and HPLC were used to determine eight of these drugs in spiked plasma samples. Both methods allow separation of most of the compounds in a single run and are specific, sensitive, and reproducible both for therapeutic monitoring and for pharmacokinetic studies in man and animals.