Background: Liver cancer is otherwise known as Hepatic cancer which originated from the liver. Hepatocellular carcinoma (HCC) is the common primary Liver cancer and is one of the emerging problems worldwide. Very few treatments are available to treat HCC, because the molecular mechanism and other HCC mechanisms are still unclear. Cyclooxygenase 2 (COX-2), one of the most promising targets for Hepatocellular Carcinoma and it is one of the dimeric enzymes which convert Arachidonic acid into Prostaglandin H2 in the step of Prostaglandin biosynthesis. Several, natural bioactive compounds involved in the treatment of various types of cancer. Tangeretin, one of the natural polymethoxyflavone which is present in the peel of citrus fruits and it also acts as an Anti-oxidant modulator and Anti-cancerous. Methods: Computational approaches like Molecular docking and Molecular dynamics was performed to identify the potential inhibitor for Hepatocellular Carcinoma. Objectives: The main objective of this study is to find a suitable inhibitor for Hepatocellular Carcinoma. Results: In this study, COX-2 considered as the potential target for Hepatocellular Carcinoma which was examined with Tangeretin. Conclusion: Tangeretin was screened against C0X-2 which includes Molecular Docking, DFT analysis, ADMET prediction, and Molecular Dynamics simulation. Tangeretin had good docking scores, fulfill the pharmacological properties through ADMET prediction, and the Protein-Ligand complex had good stability in Molecular Dynamics simulation.

Aim: Identification of promising Breast cancer inhibitors through in vitro and in silico studies. Background: Piper betel. L. is a traditional herbal, used for varied ailments. Objective: The present investigationis designed to evaluate the anti-carcinogenic potency of HC against MCF-7 cell line using in vitroanalysis. Furtherin silicoexamination to detect and formulate protein-ligand complex of HC using Molecular Docking technique. Methods: In vitro study was conducted, using MTT assay and microscopic examinations to determine the cell viability and morphological demolitions on MCF-7 cells. In silico,scrutinizes were performed, using virtual screening, docking, ADME, DFT analysis, MMGBSA, and Molecular dynamic simulation to appraise hydroxychavicol stability. Results: HC showed an outstanding anticancer potential, with dose and time dependant patterns in MTT assay and through the fluctuating organization of MCF-7 cells. Insilico analysis, results showed that the selected lead compound-complex exhibited good stability and a highly potent inhibitor to the target breast cancer protein. Conclusion: This study is evident to consider HC as an alternate potential inhibitor.

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Introduction: Hepatocellular Carcinoma (HCC) is considered one of the deadliest cancers which grow rapidly worldwide. Fucoxanthin can be acquired from edible brown seaweeds, reported that fucoxanthin has numerous physiological functions and biological abilities, and various medicinal properties. Aim: To evaluate the anti-carcinogenic efficiency of fucoxanthin against the Human Hepatoma Cell Line (HepG2). Materials and Methods: This in-vitro examination and research was carried from June 2018 to August 2018, at Alagappa University, Karaikudi, Tamil Nadu, India. Fucoxanthin at different concentrations was taken to demonstrate its antiproliferative potential and its cytotoxic effect. In this present examination, based on the outcome of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl2H-tetrazolium bromide (MTT) assay, 80 µM and 100µM were chosen for further in-vitro studies. It was noted that remarkable leakage of Lactate Dehydrogenase (LDH) and a noteworthy decrease in the Glutathione (GSH) content of 80µ M and 100µM of fucoxanthin treated HepG2 cells. Under light and fluorescence microscopic examination, there was a significant reduction in cell growth and cell proliferation in the fucoxanthin-treated HepG2 cells. Data were presented as the mean±standard deviation. The one-way analysis of variance (ANOVA) and Tukey’s multiple comparison method were used to find the level of significance. Results: The human HepG2 to be studied and analysed were divided into group I: control group; group II: comprising those cells treated with fucoxanthin in concentration of 80 µM; group III: comprising those cells treated with fucoxanthine in concentration of 100 µM. The results of the MTT assay put forward that fucoxanthin has the cytotoxic and anti-proliferative potential against HepG2 further fucoxanthin significantly alter the marker enzymes level (p-value <0.05). The therapeutic efficiency of fucoxanthin might be due to the antioxidant effect of the bioactive compound. In this present investigation, the results of in-vitro studies, fucoxanthin strongly put forward that it has potential anticarcinogenic efficacy against the hepatic tumour (p-value <0.05). Conclusion: The results of this analysis undoubtedly symbolise that fucoxanthin has anti-oxidant activity and anti-carcinogenic efficacy against primary liver cancer HCC. Due to their therapeutic efficacy, may be considered as an excellent candidate against HCC.

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