ABSTARCTBacteriocin like substance with antimicrobial activity was purified from freshly collected rumen liquor using 60% ammonium sulphate precipitation followed by ion exchange(SP-Sepharose) and gel filtration (Sephadex G25) chromatographic techniques. Purity of the product was checked on SDS-PAGE, having molecular weight of 6.5 kDa. Anti-microbial activity was demonstrated using Bacillus subtilis by gel overlay method and agar cut well diffusion method. Proteomic analysis confirmed the substance as bacteriocin. The purified sample was resistant to the action of protease. The substance was active at pH 4, 7 and 10. It was also active at autoclave temperature. The peptide purified was found to inhibit the growth of Staphylococcus aureus (MTCC87), Listeria monocytogenes (MTCC 657) and Pseudomonas aeurginosa (MTCC 424).

No abstract

Forty five Staphylococcus aureus isolated from cases of bovine mastitis were subjected to Molecular typing by Polymerase chain reaction to determine their capsular polysaccaharide type. Of the 45 isolates, 33 were confirmed to carry a cap5 locus and cap8 locus was detected in remaining 12 isolates. To the best of our knowledge this is the first report of capsular polysaccharide typing of S.aureus isolates from India

Isolation and Molecular Characterization of Methicillin-Resistant Staphylococcus pseudintermedius (MRSP) in dogs were carried out. A total of 25 ear swabs were collected aseptically from both diseased (otitis externa) and apparently healthy dogs (for vaccination) presented to teaching Veterinary Clinical Complex, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Puducherry. On analysis of 25 ear swab samples, a total of 14 (56%) Staphylococcus pseudintermedius (S. pseudintermedius) isolates were recovered, 6 (24%) isolates from healthy, and 8 (32%) isolates from diseased dogs. Among the 14 S. pseudintermedius isolates, eight isolates for mecA, which is 21.40% (3/14), and 35.70% (5/14) of MRSP isolates were detected in healthy and diseased dogs, respectively. The present study highlighted the presence of MRSP in dogs in Puducherry region.

No abstract