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A simple and rapid sample preparation procedure followed by RP-HPLC with UV detection was optimised and developed for simultaneous quantification and identification of three plant hormones namely, IAA, GA(3) and ABA in the liquid biofertilisers. This procedure allows quantification of the three plant hormones in their natural states without any prior derivatisation step. The results presented illustrate that the contents of the three plant hormones depended on the type of fruit wastes, fermentation time and the number of microorganisms found in liquid biofertilisers. This method can be extended to determine the quantity of three hormones in other matrices. This assay procedure will aid in the development of liquid biofertilisers, a valuable alternative fertilisers to promote plant growth. This process will help farmers to reduce production cost and pollution problems.

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ABSTRACT:The effect of pyoverdin I, a natural product from a Thai Pseudomonas aeruginosa strain, on the solubilization of iron and copper in two different artificially contaminated soils was investigated. The mobilized concentrations of both metals in the samples were determined by atomic absorption spectrometry. From these results, the presence of pyoverdin I can significantly increase the amount of extractable iron and copper compared to the control. The adsorption and desorption of these metals depend not only on the environmental pH but also on the soil type, which results from differences in the quantity of the organic matter. The ability of pyoverdin I to mobilize either metal in the soils indicates its important role in connection with toxicology, as well as for soil extraction and weathering. Studying the mobilization of metals by the bacterial pyoverdin I as a function of pH may provide additional information on the chemical association of metals in soil.