Optimised separation procedures for the simultaneous assay of three plant hormones in liquid biofertilisers

Abstract

A simple and rapid sample preparation procedure followed by RP-HPLC with UV detection was optimised and developed for simultaneous quantification and identification of three plant hormones namely, IAA, GA(3) and ABA in the liquid biofertilisers. This procedure allows quantification of the three plant hormones in their natural states without any prior derivatisation step. The results presented illustrate that the contents of the three plant hormones depended on the type of fruit wastes, fermentation time and the number of microorganisms found in liquid biofertilisers. This method can be extended to determine the quantity of three hormones in other matrices. This assay procedure will aid in the development of liquid biofertilisers, a valuable alternative fertilisers to promote plant growth. This process will help farmers to reduce production cost and pollution problems.

A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes

“…In previous studies, several sample pretreatment methods have been developed. These methods generally involved liquid extraction with different acid or alkaline solvents and further purifications via solid phase extraction (SPE) with a wide variety of sorbents, i.e., reversed-phase, immunoaffinity or polymeric materials [6][7][8][9]. However, most of SPE cartridges mainly targeted several compounds with similar chemical properties for multiresidue analysis [10,11].…”

Highly sensitive and quantitative profiling of acidic phytohormones using derivatization approach coupled with nano-LC–ESI-Q-TOF-MS analysis

“…Many reports have described the MSbased analysis of endogenous phytohormones [7][8][9][10]. However, the MS-based methods still have been restricted to achieve satisfactory ionization efficiency for negatively charged acidic phytohormones, which is mainly due to the low MS response of acidic analytes [11,12], serious matrix effect [13,14], and interference among analytes [15].…”

Determination strategies of phytohormones: recent advances

“…exchange ability, is well exploited for a rapid and effective purification process, 25,27,[32][33][34] and it is in good conformity with the analysis of multiple phytohormones which present diverse structural characteristics. Besides, it is reported that the mixedmode cartridges are able to significantly decrease the ion suppression effects of the plant matrix in MS detection.…”

“…Therefore, LC coupled with various types of detectors is the most commonly applied method for hormone determination. 29,34,[46][47][48] Two-dimensional (2D) LC has been employed in the quantification of plant hormones. Dobrev et al 33 developed a 2D-LC method in 'heart cutting' mode, which efficiently removed the coeluting contaminants with indole-3-acetic acid (IAA) and ABA.…”