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Manjisthadi churna has been traditionally used in the Ayurvedic system of medicine and by traditional medical practices of India to treat hyperlipidemia. A rapid, simple and accurate method with high performance thin layer chromatography has been developed to standardised Manjisthadi churna using rubiadin, sennoside and ellagic acid as markers. Methanol extract of Manjisthadi churna were used for high performance thin layer chromatography on silica gel plates. The Rf of rubiadin, sennoside-A and ellagic acid were found to 0.48, 0.23 and 0.72, respectively with densitometric scanning at 280 nm and the calibration plot were linear in the range of 100-600 ng of markers. The correlation coefficients were higher than 0.99 were indicative of good linear dependence of peaks area on concentration. The rubiadin, sennoside-A and ellagic acid contents in Manjisthadi churna were found to be 0.014, 0.038 and 0.534% w/w, respectively. This method permits reliable quantification of rubiadin, sennoside-A and ellagic acid with good resolution and separation of the same from other constitutes of the extract of Manjisthadi churna. Recovery value from 95.66-102.33% showed the reliability and reproducibility of the method. The proposed high performance thin layer chromatography method for simultaneous quantification of markers in Manjisthadi churna can be used for routine quality testing.

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Heterosis breeding has proven to be a potential method of increasing yield in the crops. It enables early identification of superior/potential crosses which is necessary to handle the material in advance generations, effectively and gainfully. An experiment was conducted to evaluate 35 genotypes in four different environments at Navsari Agricultural University. The experimental material consisted of 35 genotypes; representing 24 hybrids developed in line x tester mating of 10 diverse parents and commercial hybrid check 'OH-102'. Significant and high estimates of standard heterosis were observed for fruit yield/plant in all environments. Among the hybrids, AOL-16-04 x Parbhani Kranti was consistent with respect to higher heterotic effect for fruit yield/plant across the environments, while cross AOL-16-04 x Arka Anamika was consistent for heterotic effects in environments E1, E2 and E3 and the cross NOL 17-09 x Arka Anamika was consistent in E1, E3 and E4 for fruit yield/plant. These crosses may be exploited for commercial cultivation and may also be advanced as those would likely yield superior transgressive segregants.