The present work deals with the removal of Brilliant Green dye from wastewater using a poly(acrylic acid) hydrogel composite (PAA-K hydrogel) prepared by incorporation of kaoline clay. The composite has been synthesized using ultrasound assisted polymerization process as well as the conventional process, with an objective of showing the better effectiveness of ultrasound assisted synthesis. It has been observed that the hydrogel prepared by ultrasound assisted polymerization process showed better results. The optimum conditions for the removal of dye are pH of 7, temperature of 35°C, initial dye concentration of 30mg/L and hydrogel loading of 1g. The extent of removal of dye increased with an increase in the contact time and initial dye concentration. A pseudo-second-order kinetic model has been developed to explain the adsorption kinetics of dye on the PAA-K hydrogel. Thermodynamic and kinetic parameters indicate that the adsorption process is spontaneous in nature and the PAA-K hydrogel prepared by ultrasound process is a promising adsorbent compared to conventional process. The obtained adsorption data has also been fitted into commonly used adsorption isotherms and it has been found that Freundlich as well as Langmuir adsorption isotherm models fits well to the experimental results.
Background: Varied literature is documented exploring the relationship between ABO blood group and prevalence of oral and dental diseases. The aim of this study was to investigate the correlation of periodontal disease with "ABO" blood groups and Rhesus factor. Materials and Methods: A total of 684 systemically healthy subjects who were non smokers were selected by chance. Subjects with known blood group who had at least 20 teeth, were included in the study and the blood groups were confirmed from their medical records. Based on the periodontal parameters like clinical attachment loss (CAL) and bleeding on probing (BOP) the subjects were divided into three groups: healthy, gingivitis and periodontitis. The percentage distribution of ABO blood groups and Rhesus factor among the groups was tabulated. Results: There was an increased prevalence of gingivitis in subjects with blood group 'A' and periodontitis in subjects with blood group 'O', while subjects with blood group 'B' had healthy
In healthcare system, the development and modernization of phytomedicine in phytochemistry play a central and important role in drug development. Isolation and purification of bioactive compounds from naturally occurring substance is of prime importance. A sensitive and reliable high performance thin layer chromatography method has been developed to estimate gallic acid in ethanolic extract of Eulophia ochreata Lindl. Ethanolic extract prepared as applied on silica gel G 60 F254plate. The plate was developed using Toluene: Ethyl acetate: Methanol: Formic acid(6:6:0.4:1.6) as a mobile phase and detection and quantification were performed by densitometric scanning at 275nm. The system was found to give well resolved bands for alcoholic extract having Rf values as 0.8 was matched with the standard Rf values like 0.82. The densitometric chromatogram of HPTLC fingerprint of the alcoholic extract, isolate and standard gallic acid was obtained. The calibration curve of gallic acid was linear over a concentration range (0.2- 2 microg/ml) with a good correlation coefficient (R2= 0.9986) and coefficient of variation as CV- 2.4663%. The method was validated for linearity, precision, specificity and it was found to be precise, reliable, and suitable. The proposed method is simple, rapid, precise and accurate. The method was suitable for qualitative and quantitative analysis of gallic acid in the alcoholic extract of Eulophia ochreata Lindl.
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