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Physic nut or Jatrophacurcas is the only one which is an attractive species in Genus Jatrophaas an alternative to biodiesel. The big problems of J. curcas are their low production, non-homogenous fruit ripening and low genetic diversity. Therefore, this research focused on developing 14 chloroplast microsatellite (cp SSR) markers for genetic diversity study of 17 Jatropha accessions including 12 of Jatrophacurcas and 5 of the other species. However, there are only 4 primers i. e., JaCpSSR_2, JaCpSSR_5, JaCpSSR_10 and JaCpSSR_11 which generated polymorphic bands and produced 25 alleles by fragment analysis. The dendrogram was constructed by NTSYSpc-2.20k, UPGMA method and the similarity index ranged from 0.68-1.00 which separated the samples into 3 groups. The group I consisted of all samples of J. curcas which accessions from Lampang was closely related to Chiangmai and Loey. Similarly, J. curcas from Lamphun and Khonkaen with the similarity index was 1.00. Group II was J. integerrima (1), J. integerrima (2), J. multifida and J. gossypifolia formed a group together and group III was J. podagrica.

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