Grapes and red wine prepared from Vitis vinifera L. contain a variety of polyphenols. Some information is available about the polyphenols of the seeds and leaves of grapevine, but considerably less is known about the polyphenols of woody stems. In this paper, we describe the results of a study of polyphenolic compounds in grapevine stems. We demonstrate how a combination of reversed phase high-performance liquid chromatography with ultraviolet-diode array detection and electrospray ionization-tandem mass spectrometry ion-trap detection enables characterization of a phytochemical mixture of considerable complexity. As the polyphenol source, the stems of three frost-hardy grapevine varieties [Hasaine (Hasansky) sladki, Zilga, and Yubilei Novgoroda] were used. The main group of methanol-extractable polyphenols of stems consists of trans-resveratrol and its derivatives including oligomers and glucosides. As minor components of the extract, stilbenoid piceatannol as well as a number of nonstilbenoid polyphenols, mostly flavan-3-ols and phenolic acids glucosides, were determined. The total polyphenol content of the grapevine stems depends on the variety, whereby the stems of cultivar Yubilei Novgoroda with white grapes contain significantly less of both groups of polyphenols.

Field experiment with 8 different organic fertilizers (cattle dung with and without litter, pig slurry, peat compost formed from hen and pig slurry, sawdust-duck manure, noncomposted peat and straw litter with pig slurry) was carried out on loamy brown podzoluvisol since 1985.The organic fertilizers were applied in the spring of 1985 on two backgrounds: with and without mineral fertilizers. The material is based on the data of four years (1985)(1986)(1987)(1988). The arrangement of crops was the following: potato, spring wheat, barley and early barley.On both backgrounds cattle dung with litter turned out to be the best organic fertilizer, while noncomposted peat manure had a low direct and residual effect. However, before the application it must be composted with pig or hen slurry. The effect of peat composts was dependent on the background of mineral fertilizers. Pig slurry had a low residual effect and acted only as a liquid nitrogen fertilizer. The joint application of straw and pig slurry can be a suitable opportunity for dimmishing their undesirable effects.

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