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Biomass and neutral lipid accumulation were examined in Chaetoceros muelleri var. subsalsum and Navicula saprophila using a multivariate, fractional factorial design. Variables included were conductivity, temperature, nitrogen concentration, silicon concentration, time (culture age), and alkalinity. Measured characteristics included nile red fluorescence (as a measure of neutral lipid content) and ash-free dry weight (AFDW). Nitrogen concentration was the variable with the greatest effect on neutral lipid and ash-free dry weight accumulation over the ranges tested. Increasing conductivity in the range examined had a significant, negative impact on neutral lipid accumulation in both of these strains, while increasing alkalinity had a positive effect on lipid and ash-free dry weight in both strains. Experimental designs such as those described here have great potential utility in biological systems with complex interactions.

SUMMARYStudies of the primary structures of polysaccharides of growing plant cell walls have shown that these structures are far more complex than was anticipated just a few years ago. This complexity can best be appreciated by considering xyloglucan, a hemicellulose present in the cell wall of both monocots and dicots, and rhamnogalacturonan II (RG-II) and rhamnogalacturonan I (RG-I), two structurally unrelated pectic polysaccharides. This realization led us to postulate that cell wall polysaccharides have functions beyond determining the size, shape and strength of plants. Some years ago we demonstrated that oligosaccharide fragments of a branched /3-linked glucan of fungal cell walls can elicit the production of phytoalexins (antibiotics) in plants by inducing the formation of the enzymes responsible for synthesis of the phytoalexins. It has now been ascertained and confirmed by synthesis that the elicitor activity resides in a very specific hepta-/3-D-glucoside. The heptaglucoside has been shown to elicit phytoalexins by activating the expression of specific genes, that is, by causing the synthesis of the mRNAs that encode the enzymes that synthesize phytoalexins. In other words, complex carbohydrates can be regulatory molecules. Further experi ments established that oligosaccharide fragments of polysaccharides, produced by acid or base hydrolysis or by enzymolysis of primary cell walls of plants, also evoked defence responses in plants. Subsequently, we learned that defined fragments of polysaccharides, released from covalent attach ment within plant cell walls, can function as regulators of various physiological processes such as morphogenesis, rate of cell growth and time of flowering and rooting, in addition to activating mechanisms for resisting potential pathogens. Examples of plant oligosaccharides with regulatory properties (called oligosaccharins) will be described. IN TROD UCTIONMolecules of extraordinarily complex structure are present in the walls that sur round growing plant cells, the so-called primary cell walls. These structurally com plex molecules are the polysaccharides and glycoproteins that constitute more than half the mass of primary cell walls. Present studies of primary cell walls still very much involve elucidating the primary structures of the component polysaccharides and glycoproteins. This paper summarizes the current knowledge of the structures of the primary cell wall polysaccharides that are being characterized in our laboratory. We also present here a summary of evidence we have obtained in support of the hypothesis that oligosaccharide fragments of cell wall polymers act as regulatory molecules. This evidence includes the observation that pure oligosaccharides can act as potent and specific regulators of gene expression. Oligosaccharides with regulatory activities are called oligosaccharins.