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One hundred cattle-calves out of which 58 female and 42 male cattle-calves were screened for Theileria annulata infection during October-2015 to September-2016

Cyclophosphamide is used for the treatment of cancer cells and for suppressing the immune system during implantation. It adversely affects the cancer cells as well as healthy cells and increases the oxidative stress in these cells so cellular proteins, lipids and nucleic acids get denatured. It also produces liver, spleen and bone marrow toxicity. Medicinal plants such as Carica papaya and Andrographis paniculata possess hemopoietic, thrombopoietic and hepato-renal protective properties. The current study aimed to study the phytoconstituents in the hydro-ethanolic extracts of Carica papaya leaves and Andrographis paniculata plant material and their hepatoprotective effect in cyclophosphamide induced male Wistar rats.The collected plant materials were washed, dried, pulverized into fine powder, stored in air-tight containers and subjected to quantitative estimation of phytoconstituents present in the hydro-ethanolic extracts of Carica papaya leaves and Andrographis paniculata plant material. The hepato-protective properties of hydro-ethanolic extracts of these plants were evaluated on the basis of serum biochemistry analyses. Total phenolic content, alkaloids, flavonoids, saponin and cardiac glycosides were present in higher amount in hydroethanolic extract of Carica papaya leaves and was found more hepatoprotective @ 200 mg/kg b. wt. compared to Andrographis paniculata.

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