Considering the importance of drinking water and its effect on broiler performance, drinking water quality was studied using six different litter materials. The presence of coliform bacteria and Escherichia coli was investigated. The following litter materials were used in the trial: wood shavings, rice husks, chopped Napier grass (Pennisetum pupureum), 50% sugarcane bagasse (Saccharum L.) + 50% wood shavings, 50% sugarcane bagasse (Saccharum L.) + 50% rice husks, and plain sugarcane bagasse (Saccharum L.). A number of 1620 Ross ® one-dayold chicks were reared in 54 pens measuring 4.5 m 2 each, equipped with a bell drinker and a tube feeder. Water samples were collected in sterile tubes on days 28 and 42 of the rearing period, and submitted to the laboratory for analyses. Microbiological data were organized by classes expressed in a logarithm scale, where the lowest contamination corresponds to class 1 and the highest contamination to class 4. Results showed that total coliform contamination was higher on day 28 than in the end of the rearing period, and that E. coli presence was detected during both analyzed periods. The litter materials that presented lower degree of water contamination, predominantly class 1, were sugarcane bagasse and 50% of sugarcane bagasse and 50% of rice husks. INTRODUCTIONPoultry production has grown in Brazil, and since 2007 an average of 10 million tons of chicken meat are annually produced per year, with approximately 70% sold in the domestic market and 30% as exports (UBA, 2008). Broiler production technology has improved during recent years, allowing production growth within the same built area (Proudfoot et al., 1979;Santos et al., 2000;Santos et al., 2005).The aim of using good litter material in broiler production is to prevent the direct contact of the bird with the floor and to promote the absorption of the fecal moisture. The use of appropriate litter also helps maintaining thermal environment balanced as it may reduce fermentation heat production in the rearing environment (Oliveira & Carvalho, 2002). Several materials have been used as litter, such as wood shavings and rice husks, and other materials, such as paper, peanut hulls, and sand have been studied as alternatives to reduce the use of wood and help preventing lumbering (Hernandes et al., 2002;Santos et al., 2000).Animals need to consume sufficient water to satisfy their requirements. However, more important than water quantity is water quality, as the hygienic and physical-chemical quality of drinking water plays a key role in ensuring efficient animal production (Amaral et al., 1999;Li, 2009). Water is involved in every aspect of poultry metabolism and plays an important role in body temperature regulation, food digestion, and body waste excretion (Valias & Silva, 2001 (Abbas et al., 2008;Li, 2009). Bacteria in drinking water may induce health problems, and consequent lack of welfare (Koelkebeck et al., 1999;Manning et al., 2007). Moreover, contaminants in drinking water can leave residues in animal produc...

The Brazilian Ministry of Agriculture (MAPA) regulations establish 12 hours as the maximum pre-slaughter fasting period for broilers; however, many processing plants have considered this time is not sufficient, and consequently return the birds to the farms, with consequent economic losses and welfare problems. Therefore, it is necessary to investigate the possible effects of longer pre-slaughter fasting times. The objective of the present study was to evaluate the effect of pre-slaughter fasting times longer than those established by MAPA on broiler welfare, breast meat quality, and intestinal integrity. Forty 42-d-old broilers were submitted to different pre-slaughter fasting times: group I: 6 hours, group II 9h, group III 12h, and group IV 15h. Bird welfare was assessed before slaughter. After sacrifice, intestinal samples were collected to assess their morphology and morphometrics, and the Pectoralis major muscle was analyzed for pH and color. There was no influence (p>0.05) of treatments on breast muscle pH or color.There were no significant changes in intestinal morphometrics (p<0.05). Bird behavior was affected (p<0.05), suggesting that welfare was impaired as fasting time increased, but no differences in the analyzed parameters were detected between broilers fasted for 12 or 15 hours. It was concluded that the behavioral differences between birds fasted for 12 and 15 hours are not sufficient to assert that those fasted for 15 hours were in worse welfare conditions.

This study was carried out at Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu-SP, Brazil. The aim was to establish the normal values of bone mineral density (BMD) expressed in millimeters of aluminum in the tibia of broiler chickens using optical densitometry of radiographs. Four hundred Cobb male chicks were reared from 1 to 40 days of age, when 40 of them were selected and the right femur-tibia articulation was radiographed. Radiographs were taken with the X-ray equipment calibrated for 45 kvp and 3.2 mAs and a focus-to-film distance of 90 cm. An aluminum phantom ASTM-6063 consisting of 20 ladder steps with graduate density was placed parallel to the area to be radiographed and used as a densitometry reference standard. Radiograph images were analyzed using the software ATHENA - SIA. The proximal growth plate of the right tibia epiphysis was used as the standard reading region. The inclination axis of the reading window was 0 and the window was 10 mm high and 40-45 mm wide, depending on the bone size. Optical densitometry values of the radiographs ranged from 1.46 to 1.77 mmAl, and the coefficient of variation was 9.93%. It was concluded that densitometry values beyond the range established in the present study might indicate the presence of bone alteration in the tibia of broilers