A high-performance liquid chromatographic method was developed, validated and applied for the determination of hydrochlorothiazide in human plasma. The effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers on retention of hydrochlorothiazide and internal standard were investigated. The method involves solid-phase extraction on RP-select B cartridges followed by isocratic reversed-phase chromatography on a Hibar Lichrospher 100 RP-8 column with UV detection at 230 nm. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from spiked human plasma samples. Limit of quantification was 10 ng mL(-1). The method has been implemented to monitor hydrochlorothiazide levels in patient samples.

Amifostine is a relatively new organic cytoprotective drug that is actually a prodrug aimed at reduction of cumulative renal toxicity induced by cisplatin repeated administration. The mechanism of amifostin nephroprotective effects has still not been clarified, but it is considered that this medicine has direct cytoprotective effect on kidney tubuli. The main aim of this study was to reveal the effect of amifostin in prevention of nephrotoxicity induced experimentally with long-term administration of cysplatin at dose of 2 mg/kg/b.w./per week during a period of 8 weeks. The results obtained have shown that amifostin, although not completely, but largely reduces nephrotoxicity induced with long-term administration of cisplatin.