Development of solid‐phase extraction method and its application for determination of hydrochlorothiazide in human plasma using HPLC

Abstract

A high-performance liquid chromatographic method was developed, validated and applied for the determination of hydrochlorothiazide in human plasma. The effects of mobile phase composition, buffer concentration, mobile phase pH and concentration of organic modifiers on retention of hydrochlorothiazide and internal standard were investigated. The method involves solid-phase extraction on RP-select B cartridges followed by isocratic reversed-phase chromatography on a Hibar Lichrospher 100 RP-8 column with UV detection at 230 nm. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from spiked human plasma samples. Limit of quantification was 10 ng mL(-1). The method has been implemented to monitor hydrochlorothiazide levels in patient samples.

A Validated LC Method for the Quantitation of Cefotaxime in pH-Sensitive Nanoparticles

“…The total running time for the samples was 10 min. No peak from possible degradation products was observed in the chromatograms, as mentioned in other studies [31], showing the purity and stability of cefotaxime sodium in the studied conditions. The absence of any peak or interference at the elution point of cefotaxime rendered the method specific.…”

Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

“…Spectroscopic [8][9][10], High Performance Liquid Chromatography (HPLC) [11][12][13], Thin Layer Chromatography (TLC) [14], Electrochemical [15][16][17] and mass spectrometry [18] methods for estimation of MOX alone or in combination with other drugs in pharmaceutical preparation have been reported. Hydrochlorothiazide alone or in combination with other drugs is estimated by HPLC [19][20][21][22][23][24][25][26][27][28][29], TLC-densitometry [30][31][32][33][34][35][36], capillary electrophoresis [37][38][39], electrochemical [40][41][42][43] and spectroscopic methods [44][45][46][47][48][49][50][51][52][53]. Simultaneous estimation of MOX and HCT has been reported by RP-HPLC [54,…”

Development and Validation of TLC-Densitometry Method for Simultaneous Estimation of Bisoprolol Fumarate and Hydrochlorothiazide in Bulk and Tablets

“…Various analytical methods such as HPLC [9][10][11][12], differential pulse voltameter [13], stability indicating determination [14], photometric determination of HCTZ [15] were reported. BF is official in USP [16], whereas HCTZ is official in USP, IP [17] and BP [18].…”