Pineapple rind is a by‐product of the pineapple processing industry and contains nutrients and other compounds which must be utilized as a bioresource for socio‐economic benefits while preventing the potential problems of improper agroindustrial biomass disposal methods. Pleurotus ostreatus is an edible oyster mushroom with medicinal properties and can be cultivated on various agroindustrial biomass, including sawdust containing supplements. Pineapple rind was powdered and used as a supplement of composted sawdust at 2%, 5%, 10%, 12%, 15%, and 20% (w/w) on dry weight basis. A control treatment consisted of composted sawdust supplemented with rice bran at 12% (the most utilized composition in Ghana). P. ostreatus strain EM‐1 was cultivated on these treatments. Factors investigated included the spawn run period, yield, fruiting body weight and size, biological efficiency, and nutritional composition (proximate composition and Copper, Zinc and Lead content) of fruiting bodies harvested from selected high‐yielding treatments and the control treatment. Full colonization of all treatments occurred by the 34th day of incubation. Enhanced yield, fruiting body weight and size, and biological efficiency were generally recorded with supplementation at lower concentrations (2% and 5%) compared to treatments supplemented at higher concentrations. There was also a supplement concentration‐dependent alteration of the nutritional composition of the mushroom. Powdered pineapple rind can be utilized as an organic supplement at relatively low concentrations in composted sawdust for P. ostreatus strain EM‐1 cultivation. The use of lower concentrations of powdered pineapple rind in composted sawdust is advantageous as relatively less input will be required to produce higher P. ostreatus strain EM‐1 yields. Utilization of pineapple rind for mushroom cultivation will extend the pineapple plant value chain, intensify mushroom production in a sustainable way, and minimize agricultural losses.
The present study was undertaken to investigate the physicochemical and microbiological qualities of melon seed powder sold in some open Ghanaian markets. Twenty-five samples of powder were collected randomly from each of four major markets and analyzed for moisture, pH, total ash, acid insoluble ash, and free fatty acids (FFA) using standard methods. The microbial population was determined using the pour plate method. Melon seed powder samples had a neutral pH (6.9-7.3) and contained significant amounts of ash (0.1-0.6%). Acid insoluble ash of powder from one market was high (0.6%) and possibly indicate contamination with siliceous earth material. FFA ranged between 4.1 and 11.6% for powder from the four markets. Levels of lead were higher (0.4-0.8 ppm) than other metals such as cadmium (0.02 ppm) and copper (0.3-0.6 ppm). HQ values >1 were recorded for Pb, implying a greater risk of toxicity to consumers. Counts for aerobic bacteria, fecal coliforms, and yeast and molds were in the range of 3.2-4.4, 1.6-4.0, 1.4-2.8, and 1.1-3.2 log CFU/g correspondingly for these organisms. E. coli was not detected in any of the melon seed powder samples analyzed. This study highlights the need for proper handling of melon seed during processing, storage, and distribution, to safeguard its quality for consumers.
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