The aim of the research is to determine the effect of application of the developed product (extract) called ALGEX r 6 from natural mineral rock alginite in two different watering periods on the formation of root biomass and aboveground plant biomass of a selected group of medicinal plants (Melissa officinalis L., Malva verticillata L. and Ocimum × citriodorum Vis.) and determining the antioxidant activity in dried leaves and whole plants in aqueous and methyl alcohol extracts by DPPH method. The experiment was established in the Botanical Garden at the Slovak University of Agriculture in Nitra throughout 2020. ALGEX r 6 was prepared by a research team at the Slovak University of Agriculture in Nitra in the form of an extract from the natural mineral rock alginite with an application of thermal and chemical treatment. In the experiment, ALGEX r 6 was applied in the form of a watering in two variants with the same concentration of 3 % solution in 2 decilitres of water, but various application in terms of days in the pre-harvest stage of the above-ground plant biomass of 30 individual plants from each species. There are two diametrically opposite trends of ALGEX r 6 application that are manifesting themselves in M. officinalis and M. verticillata by reducing the root and above-ground part biomass compared to the control variant. The percentage proportionality of root/ above-ground part biomass in M. officinalis decreased from 62.48/30.31 % (control), to 45.57/18.85 % (variant 1) and to 36.07/17.27 % (variant 2), as well as in M. verticillata the root/above-ground part biomass decreased from 16.03/13.93 % (control), to 14.97/9.42 % (variant 1) and to 11.61/10.14 % (variant 2). In the species Ocimum × citriodorum Vis. the opposite trend manifested. The application of ALGEX r 6 watering resulted in increasing the antioxidant activity on the tested plant parts, especially in aqueous extracts in M. officinalis (from 19.30/control to 33.61 %/variant 1, 2), also in O. citriodorum (from 26.56/control to 44.16 %/variant 1), while in methyl alcohol extracts, the antioxidant activity showed a slight increase in all tested species.

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The botanical composition of 2 turfgrass mixtures on the basis of drought-tolerant species Festuca rubra L. and Festuca arundinacea Schreb. was evaluated by method of reduced projective dominance in natural conditions in the Research state Nitra (western Slovakia) during years 2007 -2012. The species composition of mixture was significant in the early stages of turf development. The rapidly evolving Lolium perenne L. (part of the mixture 1) had shortly after sowing relatively high ground cover. It was the dominant species in turf in year of turf establishment. Lolium perenne's part was successively decreased in next period. Festuca rubra agg. acquired the dominance in turf. Turfgrass mixtures, which contained slower evolving Festuca arundinacea Schreb. and very slowly evolving Poa pratensis L., had suitable ground cover in autumn in year of sowing or until spring in next year. The effect of supplied nutrients was reflected in year of sowing in mixture 1, resp. from the second year of cultivation in mixture 2, when the variants fertilized by a dose of 90 kg*ha -1 N (V3) exhibited higher ground cover of grasses compared to unfertilized variant (V1) and variant V2 (45 kg*ha -1 N). Higher dose of nitrogen (V3) promote the competitive strength of both mixtures, which resulted in low to very low occurrence of weeds in turf in comparison with other variants.

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The changes in growth rate, daily production of above-ground phytomass and lawn density of turfgrass after application of fertilizer based on polysulfide (Sulka NKS+) were evaluated in Nitra (the Slovak Republic) climatic conditions. The polysulfide preparation was applied 2-times, 4-times and 6-times during the vegetation period in 2015–2017 and the effect was compared with a zero control. Stimulatory effect of Sulka NKS+ was shown in the average daily gain of grass height and average daily gain of aboveground phytomass weight with the most visible expression in the 2nd and 3rd year of cultivation. For the thickening lawn index was found out inhibitory effect of polysulfide fertilizer. The average values of the first two characteristics (average daily gains of grass height and weight of above-ground phytomass) have also increased with the increasing number of Sulka NKS+applications.