Introduction: A total of (50) clinical specimens were collected from patients suffering from gall bladder attending to ALSadder Medical City and AL-Furat General Hospital include (gall bladder tissue, stool and blood) during the period from December 2016 to September 2017 from different age and sex. Methodology: The identification of Salmonella typhi isolates were depended on colonial morphology and biochemical tests as a primary identification. The final identification was performed with the automated VITEK-2 compact system. Result: According to the results obtained by the VITEK tests, thirty five clinical isolates of Salmonella typhi were obtained. This study revealed that totally 35 (70%) positive result include that 23 (65.7%) isolates was positive from gall bladder tissue, 11 (31.4%) from stool and 1 (2.8%) from blood. The study investigated the virulence factors encoding genes of Salmonella typhi, which play a major role in enterococcus pathogenicity such as Vi (R1), Vi (R2), ViaB , InvA, SpvC, and FimA. These genes have important role in invasion and adhesion. Conclusion: The study showed that the virulence factors of Salmonella typhi, which play a major role in their pathogenicity among gall bladder chronic infection