Present study included (50) clinical samples were collected from patients suffering from tonsillitis signs during the period from) November 2018 to January, 2019). All specimens were cultured for microscopic and microscopic study. Results show that from 50 patients, male was 60% and 40% were female. Several morphological, physiological and biochemical tests showed that P. aeruginosa constituted 18 isolates (36%) of these isolates. P. aeruginosa isolates were 18 isolates diagnosed by the morphological ,cultural and biochemical characters, the identification was confirmed by automated VITEK-2 compact system and molecular method for the presence of OprL. The results showed that only 12 (66.6%) isolates diagnosed as P. aeruginosa by automated VITEK-2 compact system and that was carrying OprL which are diagnosed as P. aeruginosa by P.C.R. According to the different diagnostic above, VITEK and PCR method were more sensitivity for P. aeruginosa detection among tonsillitis patients

Introduction: A total of (50) clinical specimens were collected from patients suffering from gall bladder attending to ALSadder Medical City and AL-Furat General Hospital include (gall bladder tissue, stool and blood) during the period from December 2016 to September 2017 from different age and sex. Methodology: The identification of Salmonella typhi isolates were depended on colonial morphology and biochemical tests as a primary identification. The final identification was performed with the automated VITEK-2 compact system. Result: According to the results obtained by the VITEK tests, thirty five clinical isolates of Salmonella typhi were obtained. This study revealed that totally 35 (70%) positive result include that 23 (65.7%) isolates was positive from gall bladder tissue, 11 (31.4%) from stool and 1 (2.8%) from blood. The study investigated the virulence factors encoding genes of Salmonella typhi, which play a major role in enterococcus pathogenicity such as Vi (R1), Vi (R2), ViaB , InvA, SpvC, and FimA. These genes have important role in invasion and adhesion. Conclusion: The study showed that the virulence factors of Salmonella typhi, which play a major role in their pathogenicity among gall bladder chronic infection