A portable fluorescence polarization imaging analyzer was developed for high-throughput fluorescence polarization immunoassay.

Binding parameters of metal complexes with albumins and DNA since the middle of 1990s are considered and summarized. The most widespread spectroscopic methods of estimation of binding parameters are discusseddirect fluorescent methods, indirect fluorescent methods (by fluorescence quenching parameters), and ways of estimation of binding constants by other optical spectroscopic methods. The methods and approaches to calculations used for the determination of binding constants are discussed. The data on the already found binding constants and kinetic parameters is systematized.KEYWORDS albumins, DNA, metal complexes, proteins, supramolecular interaction Funding information: Russian Foundation for Basic Research, Grant/Award Number: 16-03-01089_a 16-53-50027_YaF_a Abbreviations and designations used: F 0 , fluorescence intensity in the absence of a quencher; F, fluorescence intensity in the presence of a acid); NTSC, a new thiosemicarbazone; o-cbiaH, 5-(2-carboxybenzyloxy)isophthalic acid; o-van-gln, a Schiff base derived from o-vanillin and glutamine; o-van-L-met, Schiff base derived from o-vanillin and L-methionine; oxo, 5-

A simultaneous steady-state and transient photothermal-lens modality was used for both the thermal and optical parameters of aqueous dispersed systems (carbon and silica nanoparticles, metal iodides, surfactants, heme proteins, albumin, and their complexes). Heat-transfer parameters (thermal diffusivity and thermal effusivity), the temperature gradient of the refractive index, light absorption, and concentration parameters were assessed. To simultaneously measure thermal and optical parameters, the time scale of thermal lensing (characteristic time, tc) should correspond to an excitation beam size of 60–300 µm, and the relative time intervals 0.5÷5tc and (5÷20)tc should be selected for transient and steady-state measurements, respectively. Dual-beam thermal-lens spectrometers in a mode-mismatched optical schematic at various excitation wavelengths were built. The spectrometers implement back-synchronized detection, providing different measurement conditions for the heating and cooling parts of the thermal-lens cycle. By varying the measurement parameters depending on the dispersed system, the conditions providing the suitable precision (replicability, repeatability, and reproducibility) of thermal-lens measurements were found; setups with a broad excitation beam (waist size, 150 and 300 μm) provide longer times to attain a thermal equilibrium and, thus, the better precision of measurements of thermal diffusivity.