Recently the use of plant-derived extracts for the green synthesis of nanoparticles has drawn considerable attention. In the present study silver and copper nanoparticles were synthesized using extracts of Andrographis paniculata which is found to possess various pharmacological properties. The synthesized nanoparticles were characterized using UV spectroscopy, SEM with EDS, XRD, TEM and DLS. Furthermore, an attempt is made to impregnate these nanoparticles onto cotton bandages. The structure and morphology of silver nanoparticles impregnated onto the cotton bandages were confirmed by SEM. The anti-bacterial activity of cotton bandages loaded with silver and copper nanoparticles was tested against Escherichia coli , Bacillus cereus , and Staphylococcus aureus using a modified disc diffusion assay. The results indicate that the cotton bandages biofabricated with nanoparticles exhibited anti-bacterial activity in terms of zone of inhibition of growth of tested bacteria suggesting their usage as medical textiles in various biomedical applications for the prevention of infections. Hence, the nanoparticles impregnated cotton fibers can be applied for the development of masks, aprons, etc. to protect against bacterial penetration and as well to counteract the present situation of the world.