In photosynthetic systems, carotenoids play significant roles, and the prime role is light harvesting, then transferred to the photosynthetic apparatus for photosynthesis. Understanding carotenoids is a significant concern as it has a vital role in photosynthesis, and it also acts as a protective pigment in the biosystem. Carotenoids are widely distributed in almost all photosynthetic biosystems but highly accumulated in carrots, especially in (Daucus carota). Therefore, carrots can be a simpler model to understand the formation of carotenoids and their regulation with the help of exposure to sunlight. Raman spectroscopy is a noninvasive method to study molecular signature; it can be an ideal tool to understand carotenoids pigments in the growing stages of carrots. In this work, using resonance Raman spectroscopy with 532‐nm wavelength laser excitation, we were able to detect predominant carotenoid bands such as β‐carotene, α‐carotene, and lutein, at 1517–1519 cm−1 and 1521–1524 cm−1 with high peak intensities. This work gives a clear description and evidence of how light plays a profound effect on the activation and regulation of carotenoids. We observed β‐carotene in the nonphotosynthetic region of the plant, as a photoprotection of developing life is one of the significant roles of carotenoids, and Raman bands of carotenoids can be considered as the biomarker of photoprotection of developing life.

Pectin polymers are considered for lithium ion battery electrodes. To understand the performance of pectin as an applied buffer layer, the electrical, magnetic and optical properties of pectin films are investigated. This work describes a methodology for creating pectin films, including both pristine pectin and Fe-doped pectin, which are optically translucent, and explores their potential for lithium-ion battery application. The transmission response is found extended in optimally Fe doped pectin, and prominent modes for cation bonding are identified. Fe doping enhances the conductivity observed in electrochemical impedance spectroscopy (EIS), and from the magnetic response of pectin evidence for Fe3+ is identified. The Li-ion half-cell prepared with pectin as binder for anode materials such as graphite shows stable charge capacity over long cycle life, and with slightly higher specific capacity compare with the cell prepared using PVDF as binder. A novel enhanced charging specific capacity at high C-rate is observed in cells with pectin binder suggesting within a certain rate (∼5C) pectin has higher capacity at faster charge rates. The pectin system is found as a viable base material for organic-inorganic synthesis studies.

Understanding life at the molecular level is inherently difficult, as no precise method can very specifically determine “The activities of life.” The feasibility to observe the activation of life in an organism using molecular information through Raman spectroscopy in various biological systems is investigated in this work. The idea of determining the “dormant” state to the “growing state” state in complex organisms using specific Raman spectral bands is explained in this study. We found the Raman spectral evidence of carotenoids molecule that shows its appearance from freshwater worms to germinated and non‐germinated seeds. This study investigates the molecular functions of carotenoids in different biosystems. Given the complexity of biological systems, observations from Raman spectroscopic measurements indicate that carotenoids show strong molecular activity when life is growing as compared with a dormant state. We conclude that carotenoid's Raman bands can be considered as the biomarker of growing states in living organisms.