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Indirect transmission of infection through stethoscopes is a part of nosocomial infection. It can be reduced by cleaning stethoscopes with 70% isopropyl alcohol routinely. Aims and objectives i) whether stethoscope act as a vector for HAI ii) to see effectiveness of commonly used antiseptic and iii) to check the cleaning habits of health care providers of their stethoscopes . Methods-The samples were collected from 65 stethoscopes of health care personnel working in different hospitals and Inoculation were obtained by pressing the diaphragm surface firmly on blood agar plate and MacConkey's agar plate. Identification of organisms and antibiotic susceptibility testing were determined by standard conventional methods. Result-31 pathogenic organisms were isolated from 65 stethoscope diaphragm. Most commonly isolated organisms were Coagulase negative Staphylococcus (15.40%). Five out of 31 pathogenic organisms were multidrug resistant. Conclusion-Routine cleaning of stethoscopes reduces the bacterial load and it should be a part of infection control practice.

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Background: Tigecycline is the first commercially available glycycline, derivatives of the tetracycline antibiotics, having enhanced activity against various pathogens. In vitro activity has been demonstrated against multi drug resistant Gram positive and Gram negative pathogens like MRSA, ESBL producing Esch.coli and Klebsiella spp. and Acinetobacter Objective: To determine Tigecycline in vitro susceptibility pattern in MRSA, ESBL producing Esch.coli and Klebsiella spp. and Acinetobacter spp. Material and Methods: Investigations were carried out from August 2012-January 2014 to detect MRSA,ESBL producing Escherichia coli and Klebsiella spp. as well as Acinetobacter spp. by using a standard protocols and Tigecycline in-vitro susceptibility testing was done by Kirby Bauer Disc diffusion method and it's MIC value against resistant isolates was evaluated.Statistical analysis was done by Fishers extract method. Result: Out of 107 Staphylococcus aureus isolates 52(48.59%) were MRSA, none of them showed resistance to Tigecycline. Out of 82 Esch.coli isolates 14 (i.e 17.07%) were ESBL producers, none of them showed resistance to Tigecycline.Out of 67 Klebsiella isolates 21 (i.e 31.34%) were ESBL producers out of which only 1 (i.e 4.76%) was resistant to Tigecycline.Out of 19 Acinetobacter spp.isolated 3 (i.e 15.78%) were resistant to Tigecycline. The MIC range for ESBL producing Eschcoli, Klebsiella spp., MRSA and acinetobacter spp were 0.14-0.45 µg/ml,0.25-2.4 µg/ml,0.12-0.26 µg/ml, 1-3.2 µg/ml respectively. Conclusion: The results of the study confirm the excellent in vitro activity of Tigecycline against Gram positive and Gram negative multidrug resistant pathogens.