A simple, rapid and sensitive RP-HPLC method was developed for the quantitative determination of tramadol hydrochloride and paracetamol in combined tablet dosage form. The chromatographic analysis was carried out on enable C18G column (250 x 4.6 mm, 5 μm) with mobile phase containing 1 % glacial acetic acid: acetonitrile (50:50 v/v). The flow rate of mobile phase was 1.0 mL/min and effluents were monitored at 272 nm. The retention times of tramadol hydrochloride and paracetamol were 2.032 min and 2.711 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity and robustness. The method was found to simple, rapid and sensitive and was successfully applied to the estimation of tramadol hydrochloride and paracetamol in combined dosage form.

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