RP-HPLC method for estimation of tramadol hydrochloride and paracetamol in pharmaceutical formulation

Hemant, Kumar T; Krishna, Prajna C Kasi; Varaprasad, Rao K; Srinivasa, Rao Y.

doi:10.30574/gscbps.2019.8.1.0091

Cited by 6 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The literature survey revealed that many analytical methods like Colorimetry [8], UV spectrophotometry [9], HPTLC [10] and HPLC [11][12][13] were reported for the estimation of Tramadol HCl in pharmaceutical formulations, which involved lengthy sample preparations using expensive solvents. To the best of our knowledge, there is no published literature data on a solvent-free analytical method for the quantitation of Tramadol HCl in pharmaceutical dosage form using FTIR.…”

Section: Fig 1: Structure Of Tramadol Hydrochloridementioning

confidence: 99%

“…All the results show that the proposed method can be used as an alternative for the assay of Tramadol HCl capsules. It shows an easy sample treatment procedure consuming less time compared to other procedures such as colorimetry [7], visible and ultraviolet spectroscopy [8,9], HPTLC [10] and HPLC [11][12][13]. The advantage is that no organic solvent is required.…”

Section: Analysis Of Marketed Formulationmentioning

confidence: 99%

See 1 more Smart Citation

New Diffuse Reflectance Infrared Fourier Transform Spectroscopy for the Estimation of Tramadol Hydrochloride Capsules

BURELA

NAMMI

MANDALEMULA

2023

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: The objective of the study was to develop and validate a new diffuse reflectance infrared fourier transform (DRIFT) spectroscopic method for the quantification of Tramadol HCl in the pharmaceutical dosage form. Methods: The Fourier Transform Infrared (FTIR) analysis was carried out on Cary 630 FTIR spectrophotometer (Agilent Technologies, USA) equipped with a diffuse reflectance sampling interface (650-4000 cm-1, 32 scans, 8 cm-1 resolution). The solid-state samples were prepared by dilution in dry potassium bromide and were analysed by an FTIR spectrophotometer with the DRIFT sampling technique, which follows the Kubelka-Munk model depicting the theory of diffuse reflectance at scattering surfaces, relating band intensities to concentration for transmission measurements similar to Beer’s law. Results: A linear relationship was found in the selected wavenumber range of 3295-3298 cm-1denoting the carboxyl peak in the concentration range of 0.6-3.0% w/w with a good correlation coefficient of 0.997. The percent recovery of Tramadol HCl in the marketed dosage form was found to be 100.4%. Conclusion: The proposed method was found to be accurate, precise, reproducible, and eco-friendly. DRIFT spectroscopy may have the potential as an alternative method for qualitative and quantitative analysis of Tramadol HCl in its capsules.

show abstract

Section: Fig 1: Structure Of Tramadol Hydrochloridementioning

confidence: 99%

Section: Analysis Of Marketed Formulationmentioning

confidence: 99%

New Diffuse Reflectance Infrared Fourier Transform Spectroscopy for the Estimation of Tramadol Hydrochloride Capsules

BURELA

NAMMI

MANDALEMULA

2023

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

show abstract

“…It is impracticable, challenging, and more expensive to experimentally verify several factors at once. The reversed-phase technique [12][13][14][15] is the method of preference in analysis due to its ease of use, adaptability, and diversity of applications, which include handling compounds with a variety of polarities and molecular masses.…”

Section: Introductionmentioning

confidence: 99%

Utility of Quality by Design approach in RP-HPLC method development for quantification of lamivudine and effavirenz in combination formulation

Koppisetty

PRASAD

AMGOTH

et al. 2023

Ankara Ecz. Fak. Derg.

View full text Add to dashboard Cite

Objective: For the measurement of lamivudine(LAM) and effavirenz (EVZ) in combination formulation, a uncomplicated and reliable liquid chromatographic approach has been proposed. Material and Method: Multivariate optimization of the RP-HPLC method, experimental conditions were accomplished using the design of experiments (DoE). The crucial method parameters were determined by a risk assessment. The mathematical models were created using three independent variables: percentage of acetonitrile, percentage of methanol, and buffer pH. The impacts of these independent elements were thoroughly investigated using the central composite design (CCD), which was utilized to analyze the response surface methodology. Result and Discussion: The LAM and EVZ retention time and resolution were both concurrently optimized using the desirability function. Acetonitrile, methanol, and phosphate buffer (pH 7.0) in the proportions of 40:20:40 v/v each were used in the optimized and anticipated data from the contour diagram, with detection occurring at a wavelength of 215 nm. Baseline separation of both pharmaceuticals with high resolution and a run time of under 6 minutes was accomplished under these ideal conditions. The validated test parameters followed ICH recommendations. As a consequence, the findings demonstrated that the Quality by Design methodology could be successfully used to optimize the RP-HPLC technique for the concurrent quantification of LAM and EVZ.

show abstract

“…HPLC has also been developed through the stationary phases that have been improved through the 30-alkyl carbon atoms chains. A number of techniques have been described the determination of ceftriaxone in literature such as HPLC-UV [1][2][3][4], HPLC-MS [5,6], UPLC-MS/MS [7,8], spectrophotometry [9][10][11] and spectrofluorometric [12] The RP-HPLC [13][14][15][16][17][18][19][20] methods have superiority over the UV spectrophotometric methods [21][22][23][24] in terms of selectivity and sensitivity. We here in reported a simple, sensitive, precise, accurate, linear and isocratic RP-HPLC method for the simultaneous quantitative estimation of Ceftriaxone sodium as per ICH guidelines.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of new RP-HPLC method for the estimation of ceftriaxone sodium in bulk and pharmaceutical dosage form

Lavanya Sankatala,

Manjunath Sooganna Yalagatti

2023

GSC Biol. Pharm. Sci.

View full text Add to dashboard Cite

A simple, rapid, accurate and precise RP-HPLC method was developed and validated for the determination of ceftrioxone sodium in parentral dosage form. Chromatographic analysis of the drug was achieved on CYBER LAB HPLC comprising of LC- 100 P pump, a variable wavelength programmable LC-UV100 UV detector and SCL system controller. Flowrosil C18 column (250 mm x 4.6 mm, 5 μ) as stationary phase with mobile phase consisting of methanol: water : ortho-phosphoric acid in the ratio of (75 :24.5 :0.5 v/v ) . The method showed a good linear response in the concentration range of 10-50 μg/ml with correlation coefficient of 0.9990. The flow rate was maintained at 1.0 ml/min and detection was carried out at 240 nm. The retention time was 3.788 min. The method was statistically validated for accuracy, precision, linearity, ruggedness, robustness, solution stability, selectivity and sensitivity. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the determination of ceftrioxone sodium in parentral formulation.

show abstract

RP-HPLC method for estimation of tramadol hydrochloride and paracetamol in pharmaceutical formulation

Cited by 6 publications

References 8 publications

New Diffuse Reflectance Infrared Fourier Transform Spectroscopy for the Estimation of Tramadol Hydrochloride Capsules

New Diffuse Reflectance Infrared Fourier Transform Spectroscopy for the Estimation of Tramadol Hydrochloride Capsules

Utility of Quality by Design approach in RP-HPLC method development for quantification of lamivudine and effavirenz in combination formulation

Development and validation of new RP-HPLC method for the estimation of ceftriaxone sodium in bulk and pharmaceutical dosage form

Contact Info

Product

Resources

About