Association of Mycoplasma mycoides subspecies capri (Mmc) with natural goat mastitis has been studied earlier largely by detecting the Mmc DNA using molecular methods. However, report on detection of cultivable Mmc isolates from natural goat-mastitis milk is still very rare. In this study, Mmc was isolated from milk samples (n = 171) of goats with or without clinical signs of mastitis. Mmc isolates were further characterized by biochemical and species-specific PCR methods. Intra species strain variation was also studied by 16S amplified rDNA restriction analysis (16S ARDRA). The study recovered a total of 6 Mmc isolates (3.5%). Three types of intraspecies variants among the recovered Mmc isolates were found by 16S ARDRA. The study concluded that Mmc may be an etiological agent of mycoplasmal mastitis in Indian goat herds.

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This study aims to evaluate the genetic potential of the Jamunapari goat and formulate a new selection strategy for improving the lactation traits. The data set included 4049 phenotypic records for lifetime milk yield at 90 days (MY90) and 140 days (MY140), total milk yield (TMY), and lactation length (LL) obtained from the progeny of 83 sires and 1643 dams between 1990 and 2019. Animal model employing average information restricted maximum likelihood (AIREML) was used to estimate genetic parameters for milk yield traits and LL. The direct additive heritability estimates for lifetime lactation traits, that used repeatability model were 0.10 ± 0.03, 0.08 ± 0.03 and 0.12 ± 0.02 for MY90, MY140 and TMY, respectively, while it was low for LL (0.06 ± 0.02). The repeatability estimates were moderate ranging from 0.17 to 0.22 for milk yield traits and LL, indicating persistent performance over the parities. Animal permanent environment in uence (c 2 ) was signi cant in milk yield attributes, whereas additive maternal genetic effects were absent. As the early selection criteria based on rst parity records is essential, we analysed the data for the rst parity separately and obtained moderate h 2 estimates viz. 0.26 ± 0.05, 0.26 ± 0.06 and 0.25 ± 0.06 for MY90, MY140 and TMY, respectively. These estimates augurs further positive scope of selection in Jamunapari goats for higher milk yield. High and positive genetic correlation of MY90 with MY140 (0.97 ± 0.01) and TMY (0.91 ± 0.05) revealed the scope of using MY90 as the selection criterion.Based on these results, we recommend use of MY90 as a single trait selection criterion for genetic improvement of all lactation traits in Jamunapari goat.

We have identified the bioactive peptides in goat milk using two dimensional gel electrophoresis and mass spectrometry. The milk samples were collected from three healthy breeds of goats at CIRG farms. The milk proteins were isolated using simple centrifugation and were evaluated on the basis of presence of other proteins variants using SDS PAGE and two dimensional gel electrophoresis. The protein profiles of all the milk proteins were same showing the presence of all the major caseins proteins and whey proteins whereas the two dimensional gel electrophoresis resulted gels has shown the presence of various protein spots. These protein spots were analysed under mass spectrometry and found biologically active peptides dipeptidyl peptidase, transcription factor A, Interleukin 12 subunit, oligodendrocyte transcription factor 2. In our study we have reported the biological significance of these peptides identified and their role in human health such as mitochondrial disease, brain malignancies, signaling processes of cytokines and help in generating cytotoxic lymphocyte helps enhance immunity.

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