The differential interference contrast (DIC) and fluorescence confocal micrographs show collagen microfibrils attacking graphite from all sides to form a stable dispersion of collagen modified graphene, but only collagen picks up a stain.

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Indocyanine green (ICG) is one of the FDA-approved near infra-red fluorescent (NIRF) probes for cancer imaging and image-guided surgery in the clinical setting. However, the limitations of ICG include poor photostability, high concentration toxicity, short circulation time, and poor cancer cell specificity. To overcome these hurdles, we engineered a nanoconstruct composed of poly (vinyl pyrrolidone) (PVP)-indocyanine green that is cloaked self-assembled with tannic acid (termed as indocyanine green-based glow nanoparticles probe, ICG-Glow NPs) for the cancer cell/tissue-specific targeting. The self-assembled ICG-Glow NPs were confirmed by spherical nanoparticles formation (DLS and TEM) and spectral analyses. The NIRF imaging characteristic of ICG-Glow NPs was established by superior fluorescence counts on filter paper and chicken tissue. The ICG-Glow NPs exhibited excellent hemo and cellular compatibility with human red blood cells, kidney normal, pancreatic normal, and other cancer cell lines. An enhanced cancer-specific NIRF binding and imaging capability of ICG-Glow NPs was confirmed using different human cancer cell lines and human tumor tissues. Additionally, tumor-specific binding/accumulation of ICG-Glow NPs was confirmed in MDA-MB-231 xenograft mouse model. Collectively, these findings suggest that ICG-Glow NPs have great potential as a novel and safe NIRF imaging probe for cancer cell/tumor imaging. This can lead to a quicker cancer diagnosis facilitating precise disease detection and management.

Oxidized/reduced carbon dots (CDs) with tunable optical features have emerged as a new class of CDs having a common "molecular origin" but different fluorescence (FL) behaviors. In the present work, using "banana peel" as a sole carbon source followed by doping with fluorine (F), boron (B), and nitrogen (N) over CDs, banana peel-derived carbon dots (BP-CDs) were synthesized using a well-known hydrothermal synthesis method. Moreover, as-synthesized BP-CDs were further reduced to "rBP-CDs" by NaBH 4 . At post reduction, the FL performance (i.e., quantum yield) of rBP-CDs were found to be enhanced compared with the BP-CDs, along with variations in excitation and emission wavelengths. Interestingly, the optical sensing attributes of BP-CDs and rBP-CDs were varied, that is, BP-CDs selectively sense "Co 2+ with a limit of detection (LOD) value of 180 nM", whereas rBP-CDs detected Co 2+ (with an LOD value of 242 nM) as well as Hg 2+ (with an LOD value of 190 nM). To the best of our knowledge, this work presents the very first report on the modulation of CDs' sensing behavior after reduction. The modulation in the sensing behavior with the common carbon precursor and reduction paves a new possibility for exploring CDs for different commercial applications.