Background: In 1988 the US Food and Drug Administration permitted low dose methotrexate for the treatment of rheumatoid arthritis that would change the progression of the disease. Methotrexate is a folic acid antagonist and its systemic use causes numerous side effects; including hepatic toxicity. It would be preferable to deliver methotrexate by the topical route to reduce side-effects along with ease of administration and reduced dosing frequency. So, nanoparticle gel is a hopeful approach to treat rheumatoid arthritis. Objective: To develop a nanoparticles gel containing novel natural polymer-based methotrexate nanoparticles and evaluate its therapeutic potential on Complete Freund’s Adjuvant–Induced Arthritis rat model and compare it to methotrexate and dexamethasone gel. Result and Discussion: Methotrexate nanoparticles gel significantly reduced the percentage inhibition of oedema compared to methotrexate and Dexamethasone gel. The therapeutic activity of nanoparticles gel was found to be F3W2 ≥ F2W2 ≥ F1W2 ≥ F4W2 ≥ MTX gel. So, the optimized nanoparticle gel formulation F3W2 can be effective in treatment of rheumatoid arthritis. Materials and methods: The five batches methotrexate nanoparticles gel were prepared viz. F1W2, F2W2, F3W2, F4W2 and methotrexate gel for the topical application by using different concentrations of Carbopol 934 base and characterized for their evaluation parameters: Homogeneity, Grittiness, pH, Spread-ability, Viscosity determination, and Drug content studies. The arthritic potential of methotrexate-nanoparticles gel was evaluated by CoResult and Discussion: Methotrexate nanoparticles gel significantly reduced the percentage inhibition of oedema compared to methotrexate and Dexamethasone gel. The therapeutic activity of nanoparticles gel was found to be F3W2 ≥ F2W2 ≥ F1W2 ≥ F4W2 ≥ MTX gel. So, the optimized nanoparticle gel formulation F3W2 can be effective in treatment of rheumatoid arthritis.mplete Freund’s Adjuvant–Induced Arthritis rats model based on percent inhibition oedema and arthritic score. Conclusion: The developed novel nanoparticles gel formulation can be a promising alternative to existing methotrexate and Dexamethasone gel.

: In the present study, series of 2,5-disubstituted-1,3,4 oxadiazole analogues retaining pyridine moiety were synthesized (4a-j) by reacting various substituted aromatic acids and isonicotinohydrazide by using POCl3 as cycling agent. The structure elucidation of all the synthesized compounds was done by chromatographic data and the spectral data analysis. The synthesized compounds were evaluated for their in-vitro antimicrobial activity against various strains of ESKAPE pathogens. Antibacterial activity was performed against Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, while antifungal activity was assayed against Candida albicans and Aspergillus spp. The result of invitro antimicrobial studies of all the synthesized compounds revealed that compound 4d and 4f exhibited prompt activity against selected microbial strains equally compared to Cefixime and Econazole used as reference drugs.