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The effect of quinalphos (250 pg/kg i.p.) an organophosphorus insecticide treatment for 13 and 26days on the testicular steroidogenic enzymes viz. 30-Hydroxysteroid Dehydrogenase and 170-Hydroxysteroid Dehydrogenase, as well as cholesterol content and histology of the testes of the Wistar strain rats was studied. The time duration of 13 days is approximately equivalent to one cycle of the seminiferous epithelium in Wistar strain rats. Treatment of quinalphos for 13 days failed to produce any effect on the relative weights of the testes and accessory sex glands. However, significant inhibition of 3PHSD activity and increased cholesterol ]eve] in testis were observed. The rats treated for 26 days similarly showed a highly significant inhibition of the activity of both 30-HSD and 170-HSD. The relative weights of the testes and accessory sex glands were also significantly reduced. Histological examination of the testis revealed that quinalphos treatment produced detrimental changes in the seminiferous epithelium. Treatment with quinalphos for 13days produced no toxic effect with the exception of a significant increase in serum alkaline phosphatase. However, after 26days of treatment toxicity was significantly increased as reflected on serum transaminases, phosphatases and blood urea levels of rat. Present study indicated that quinalphos impairs testicular functions in rats. Der EinfluS von Quinalphos auf die testikulare SteroidbildungZusammenfassung: Anhand einer Behandlung mit 250 pg/kg Korpergewicht iiber 13 bzw. 26 rage wurde bei Wistarratten der EinfluP von Quinalphos, einem Organophosphat-Insektizid, auf die testikular steroidwirksamen Enzyme, 3-0-Hydroxysteroid-Dehydrogenase und 17-0-Hydroxysteroid-Dehydrogenase sowie auf Cholesterolgehalt und Histologie des Hodens untersucht.Das Zeitintervall von 13 Tagen entspricht ungefahr der Zyklusdauer des Keimepithels der Wistarratte. Die Behandlung mit Quinalphos iiber 13 Tage zeigte keinen EinfluP auf das relative Gewicht von Hoden und akzessorischen Geschlechtsdriisen. Dennoch wurden eine signifikante Hemmung der 3-0-HSD-Aktivitat und erhohte Cholesterolwerte im Hoden beobachtet. Die Ratten, welche iiber 26 Tage behandelt worden waren, zeigten gleichermalkn eine hochsignifikante Hemmung der 3-0-HSD und 17-0-HSD-Aktivitaten. Das relative Gewicht von Hoden und akzessorischen Geschlechtsdriisen war ebenfalls signifiiant verringert. Die histologische Untersuchung des Hodens deckte auf, dai3 Quinalphos schadliche Veriinderungen am Keimepithel verursacht. Die Behandlung mit Quinalphos iiber 13 Tage rief keinen toxischen Effekt hervor mit Ausnahme eines signifikanten Anstiegs der alkalischen Phosphatase im Serum. Die Behandlung iiber 16 Tage fiihrte zu einem signifikanten Anstieg der Toxizitiit, wie am Serumspiegel der Transaminasen und Phosphatasen sowie am Blutharnstoffspiegel festgestellt werden konnte. Die vorliegende Studie belegt, dai3 Quinalphos die testikularen Funktionen bei Ratten beeintdchtigt.

Ebola hemorrhagic fever (Ebola HF) is caused by infection with Ebola Virus. Ebola virus, a member of the family Filoviridae, causes one of the most severe forms of viral hemorrhagic fever. In the final stages of the disease, symptoms progress to hypotension, coagulation disorders, and hemorrhages, and there is prominent involvement of the mononuclear phagocytic and reticulo-endothelial systems. It is assumed that the functions of the envelope glycoprotein are likely to play important roles in the pathogenicity of Ebola virus and the interactions of some viral proteins with the immune system are likely to play important roles in the extraordinary pathogenicity of this virus. Ebola virus (EBOV) entry requires the surface glycoprotein (GP) to initiate attachment and then fusion occurs between viral and host membranes. All glycoprotein forms are encoded by gene 4 of the EBOV genome. The strain selected is VGP_EBOSU with accession number Q7T9D9 of Sudan Ebola Virus-Uganda (2000) from NCBI'S entrez database. The 3D structure of Ebola Virus Protein was generated using Homology Modelling. For a predicted evaluation Andrographolide is used (the compound needs clinical trials to prove its efficacy in treatment). The 3D structure of Andrographolide was generated and was converted to *.pdb file which now docks with the *.pdb file of Ebola Virus Protein.

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Alzheimer's disease is an incurable, degenerative, and terminal disease. It is associated with mutations in Amyloid Precursor Protein (APP), Presenilin 1 (PS1), Presenilin 2 (PS2), or Apolipoprotein E (APOE). 3D structures of these 4 proteins were generated using Homology Modeling. Active compounds of medicinal herbs-Canscora decussate, Nardostachys jatamansi and Mucuna pruriens were selected as these three herbs have properties of memory enhancement. Chemical structures of the active component of these herbs were drawn using chemsketch, combined & converted to *.pdb. The four proteins were successfully docked with Canscora decussate-Nardostachys jatamansi -Mucuna pruriens's active component combination.