No abstract

The present study carried out to investigate the bioactive chemical compounds, total polyphenol content and antioxidant potential of different extracts of the Zanthoxylum armatum leaves collected from the Nainital, Uttarakhand. The GC-MS analysis of Z. armtum leaves extract resulted in the isolation of sixty, twelve, twenty-three and nineteen phytochemical constituents in methanol, ethanol, chloroform and water extracts respectively. The leaves extracts were strongly characterized by Heneicosane, Tetratetracontane, Phytol, Fargesin, (+)-Seasmin and Paulowin.

The present study for the first time investigated the effects of Salicylic acid (SA) (50, 100 and 200 mg/L) on in vitro growth and antioxidant capacity of Berberis asiatica. SA significantly enhanced in vitro shoot multiplication at 100mg/L concentration. The result showed highest significant increase in antioxidant activity by 50 mg/L SA elicited plantlets in terms of DPPH (2,2-Di-phenyl-1-picryl-hydrazyl) free radical scavenging activity (IC50 32.42±0.51), Ferric Reducing Antioxidant Activity (292.73±2.09 AAE±SE) and Metal Chelating Activity (IC50 13.18±0.52). The Gas Chromatography-Mass Spectrometry (GC-MS) profiling revealed presence of 46, 38, 34 and 29 phytochemicals in control, SA1, SA2 and SA3 methanolic extracts respectively. Among the identified compounds, eight compounds 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl; 1,3,4,5-tetrahydroxy-cyclohexanecarboxylic acid; 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (and Phytol); hexadecanoic acid, methyl ester; 9,12-Octadecadienoic acid (Z,Z)-,methyl ester; di-n-octyl phthalate; stigmast-5-en-3-ol,(3.beta.)-or ß-sitosterol; and squalene were previously known for antioxidant potential.

The objective of the paper is to investigate the chemical composition of Zanthoxylum armatum roots methanolic extract and to evaluate the polyphenol and antioxidant potential. Gas Chromatography-Mass Spectroscopy (GC-MS) and High-Performance Liquid Chromatography -Photo Diode Array (HPLC-PDA) techniques were used to characterize chemical composition of methanolic extract of roots. The GC-MS analysis extract resulted in the isolation of thirty-seven phytochemical constituents in methanol extracts. The root extracts were strongly characterized by 1-Propene, 2-nitro-3-(1-cyclooctenyl), (2E,4E)-N-Isobutyldodeca-2,4-dienamide, (+)-Seasmin and Paulowin. The phenolic composition of the root extract quantified by using HPLC revealed Ascorbic acid as most abundant polyphenol, followed by Gallic acid, Chlorogenic acid, Epigallocatechin gallate, Vanillic acid and p-hydroxybenzoic acid, while Caffeic acid was the recorded as least. In addition, root extract also showed the presence of antioxidant activity (DPPH Free radical scavenging, Metal Chelating and Ferric Reducing Antioxidant Activity) and polyphenol content (Total Phenol, Flavonoid and Tannin Content).