The intension of present work is to incorporate economically cheap, easily available but effective herbal ingredient in personal hygiene products. Leaves of species Psidium Guajava belonging to familyMyrtaceae(Guava) have many properties like antibacterial, anti-cancer, anti-diabetic, antioxidant etc. The leaf extract of guava has traditionally been used for its health benefits. Toothpaste is a dentifrice used clean, maintain and improve the health of teeth. Toothpaste is mainly used to promote oral cleanliness and also acts as an abrasive that helps to prevent dental plaque and food particles from the teeth. The main aim of this investigation is to incorporate the herbal ingredient to that toothpaste that can effectively cleanse oral bacteria. Guava leaves were obtained from domestic garden. Guava leaves were washed with distilled water and shade dried for three days and then powdered for extraction. Guava leaf extraction was performed by Soxhlet apparatus with 70% ethanol for its antibacterial activity. This extract was used as principle ingredient for herbal toothpaste. Toothpaste formulation performed at laboratory level. The formulation was subjected to various evaluation tests like pH, spreadability, foaming ability, moisture content and zone of inhibition. All the results of evaluation tests found within the limits. For getting antibacterial property extraction is done against ethanol and agar well diffusion method used to identify its antibacterial activity shown by guava leaf extract on Escherchia coli, staphylococcus aureus depends on saponins, tannins and flavonoids. Even the extract can be used directly for treatment of inflamed gum. Pentacyclictri-terpenoidguajanoic acid is main constituent of guava leaf extract.