Hasani Q, Pratiwi NTM, Wardiatno Y, Effendi H, Martin AN, Efendi E, Pirdaus P. 2020. Phytoremediation of Iron (Fe) in Ex-sand Mining Waters by Water Hyacinth (Eichhornia crassipes (Mart.) Solms). The high concentration of iron (Fe) has allegedly caused the water quality of puddles at sand mining area. The poor water quality, in which high contentrations of iron will to a reduction in aquaculture production. This study verified the ability of water hyacinths (Eichhornia crassipes (Mart.) Solms.)) as a phytoremediation agent for iron (Fe) concentration in water at the sand mining area, in East Lampung Regency, Indonesia. The study was carried out with 3 treatments and 3 replication each, including the treatment of (i) 25% water surface coverage, (ii) 50% water surface coverage, and (iii) 75% water surface coverage of water hyacinth. Measurement of Fe concentrations, bioconcentration factor (BCF) and translocation factor (TF) once a week, until Fe concentrations in water are adequate for aquaculture. This was achieved for 21 days. The results showed that the highest percentage reduction of Fe (97.49%) was observed at water hyacinth cover area 50%. The highest value of BCF was 2385.51, while the highest TF was 1.14 in stems and 1.02 in leaves. The results of this study open up opportunities for use water in ex-sand mining areas for aquaculture by the community and its management by the Government of East Lampung Regency, Lampung, Indonesia.

<p>Abstrak<br />Penelitian ini dilakukan untuk memperoleh metode analisis dan unjuk kerja metode analisis logam mayor (Na, K, Mg dan Ca) dalam air tua(bittern) menggunakan Microwave Plasma-Atomic Emission Spectrometer. Diketahui bahwa karakteristik air tua memiliki matriks kompleks yang disebabkan oleh tingginya mineral anorganik yang terkandung didalamnya. Oleh karenanya, metode analisis yang digunakan menerapkan larutan standar multi elemen mendekati matriks air tua. Karakteristik kinerja dari metode ini ditentukan dengan uji larutan standar multielement (Na, K, Mg dan Ca), dan unspiked sampel. Selektivitas, linearitas, akurasi, presisi, deteksi dan batas kuantifikasi dari hasil analisis yang diperoleh kemudian dievaluasi. Efek matriks yang signifikan tidak teridentifikasi untuk semua elemen yang dianalisis. Linearitas (r) metode analisis untuk elemen Na, K, Mg dan Ca berkisar antara 0,9955 - 0,9998. Hasil uji akurasi menunjukkan bahwa tidak ada penyimpangan dari nilai benar untuk semua elemen yang dianalisa. Presisi yang disajikan dalam relative standard deviation (%RSD) untuk analisa Na, K, Mg dan Ca berturut-turut adalah: 1,491; 2,702; 2,678; dan 1,923. Sedangkan batas deteksi untuk parameter Na, K, Mg, dan Ca berturut-turut adalah: 0,184; 0,070; 0,219 dan 0,005 mg/l dengan batas kuantifikasi 0,613; 0,230; 0,730 dan 0,017mg/l. Hasil validasi tersebut menunjukkan bahwa metode yang diusulkan selektif dan sesuai untuk untuk analisis air tua.<br />Kata kunci : validasi metode analisis, air tua, logam (Na, K, Mg, Ca), MP-AES.</p><p><br />Abstract<br />This research aims to obtain analysis method for mayor elements (Na, K, Mg, and Ca) in bittern using Microwave Plasma-Atomic Emission Spectrometry (MP-AES) and to assess the validity of the obtained method. It is known that sea bittern characteristic has complex matrix for its high concentration of the inorganic materials. Therefore assessment of the validity of the analytical method for mayor element is important. The performance characteristics of the method were established by multi-elements-standard-solution assay (Na, K, Mg and Ca), in which samples were un-spiked. Selectivity, linearity, accuracy, precision, detection and quantification limit of the method were evaluated in the study. Matrix effects were not significantly identified for the studied elements. Linearity of selected concentration range for Na, K, Mg and Ca varied from 0.9955- 0.9998. No deviation of true value for all elements was observed in the accuracy test. Meanwhile, precision values of the method (%RSD) were 1.491; 2.702; 2.678; and 1.923, respectively for Na, K, Mg and Ca. The method developed in the present study indicated the detection limits for Na, K, Mg and Ca were 0,184; 0,070; 0,219 dan 0,005 mg/l, respectively, with quantification limits of 0,613; 0,230; 0,730 dan 0,017mg/l. The results of the validation method showed that the proposed method is selective and feasible for sea bittern analysis using MP-AES. Keywords: Method validation, Bittern, Na, K, Mg, Ca, MP-AES.</p>

Verifikasi metode analisis merupakan salah satu cara untuk memastikan bahwa suatu metode memberikan hasil yang valid dan sesuai dengan tujuan. Verifikasi metode analisis US.EPA 200.7 untuk pengujian logam berat di air telah dilakukan dengan parameter verifikasi meliputi linieritas, presisi, akurasi, batas deteksi dan batas kuantifikasi. Hasil uji menunjukkan bahwa metode mempunyai linieritas yang baik dengan nilai koefisien korelasi >0,995. Nilai presisi yang diperoleh lebih kecil dari nilai Hortwitz menunjukkan keberterimaan metode dengan rentang RSD dari 1,21 sampai 10,82%. Tehnik spiked sample telah dilakukan untuk akurasi metode uji dengan hasil persen perolehan kembali (% recovery) telah memenuhi syarat 80-100%. Batas deteksi untuk logam Pb,

Method verification for metal analysis (Cd, Cr, Cu, Ni, Co, and Mn) in surface water using Inductively Coupled Plasma (ICP) was carried out to evaluate the method’s performance in the laboratory-based on US Method EPA 200.7. The verified method is used to determine the metal content in river water flowing in Bandar Lampung. The results showed that the method used had good linearity with a regression coefficient of more than 0.995. This method’s accuracy is expressed by the %RSD (relative standard deviation), which is in the range of 3.145 to 4.345% and meets the acceptance requirements with a %RSD value less than ⅔ CV Horwitz. The method accuracy obtained from the spiking analysis gives a range of 80-110% for the analysis of 1 mg/L and meets the acceptability required by AOAC. Overall, the performance of the method used is suitable for the analysis of metals in surface water. This method was applied for metal analysis in river water samples in several places in Bandar Lampung, which were the Palang Besi river (A1), the Way Balau Kedamaian river (B1), the Way Balau Kedaton river (C1), the Way Kuala river (D1), the Sumur Batu Kahuripan river (E1), Sumur Putri river (F1), and Muara Kahuripan river (G1). The concentrations of Cd, Cr, Cu, Ni, and Co metals were under the LoD method, while the Mn concentration was above the LoD method in river water samples.