Abstrak: Salah satu peran serta masyarakat dalam mendukung program pemerintah dalam ketahanan pangan adalah memanfaatkan sumber daya alam lokal. Setiap daerah memiliki sumber daya alam berbeda-beda yang dapat digunakan sebagai pemenuhan kebutuhan pangan seperti tanaman keladi, singkong, pepaya, pisang, mangga, kangkung, dan cabai. Pemanfaatan lahan kosong disekitar lingkungan tempat tinggal dapat digunakan untuk menanam dan mengembangkan beberapa jenis tanaman tersebut. Sehingga pemenuhan gizi dari pangan akan dengan mudah terpenuhi. Pengabdian dilakukan pada tanggal 14-15 September 2019, yang berlokasi di Rumah Singgah, Desa Galiran, Kabupaten Klungkung, Bali. Tujuan dari pengabdian ini adalah menanamkan pemahaman tentang kemandirian pangan dan bagaimana mewujudkannya. Serta keuntungan bagi mereka sebagai sumber kecukupan pangan dan gizi sehari-hari. Metode yang digunakan dalam pengabdian ini tim pengabdi memberikan penyuluhan tentang pengetahuan kemandirian pangan hubungannya dengan ketersediaan pangan. Pemanfaatan lahan kosong menjadi lahan produktif untuk ditanami tanaman buah-buahan, sayuran dan keladi sebagai sumber pangan pokok, vitamin dan dapat mengurangi pengeluaran. Sebelum dan sesudah penyuluhan peserta diberikan pre dan post-test yang hasilnya menunjukkan peningkatan pengetahuan peserta melalui analisis uji Wilcoxon sebesar 0,0110,05. Beberapa bibit tanaman diberikan sebagai stimulator kegiatan menanam yang hasilnya nanti dapat dinikmati bersama. Dampak dari pelaksanaan pengabdian ini mitra memperoleh ilmu pengetahuan tentang kemandirian pangan, serta aplikasinya dalam budidaya bibit tanaman. Salah satu dampak positif penanaman bibit pisang di pekarangan Rumah Singgah, sudah menghasilkan buah pisang.Abstract: One of community participation to support government programs in resilience food, utilizes local natural resources. Each district has different types of natural resources, which use as a fulfillment of food needs, such as taros, cassavas, papayas, bananas, mangoes, kales, and chilies. The utilization of unused yards around the house can be utilized to plant and grow several types of plants. It will make it easier to fulfill food nutrition. This program was held on 14-15 September 2021 at Orphans House at Galiran village, Klungkung Bali. The aim of this program is to give knowledge about food resilience and the way to realize it. The advantages is will give them adequate of food and nutrition resource daily. The method that we used in this program was giving a lecture about the resilience of food which connected to food availability. Utilized unproductive yard to be productive by the planting of fruits, vegetables, and taro as a staple food, vitamin resource and will reduce the expenses. We have done pre and post-test before and after doing a lecture, the obtained result is 0,0110,05 which analyzed by Wilcoxon. The result showed if the knowledge of the participant has increased. Some of the plants' seeds have been given as a stimulator farming activity where they will enjoy the harvest. The positive impact to the orphans was they received knowledge about food resilience and the application by receiving plant seed. One of the greatest impacts that banana seeds which had planted, has been growing and given results.

The Covid 19 pandemic and unhealthy life style may lead to depression and anxiety followed by a decreasing of immune system. A healthy diet affects a person’s health and immune system. Objective: To provide education to the community, especially employees and lecturers in the LLDIKTI region VIII work environment, regarding food consumption and nutrition that can prevent stress and increase endurance during a pandemic. Methods: The activity begins with preparation, implementation and evaluation. Education is carried out using virtual media through zoom meetings, which are in the form of webinar series containing lectures and discussions. At the beginning of the activity, partners are given a pre-test and at the end of the discussion partners are given a post-test. Community service activities are carried out from October 13, 2021, to November 13, 2021. The evaluation was carried out using a knowledge questionnaire based on the material presented by the speaker.  The results of the community service activities showed an increase in partner knowledge about food and nutrition consumption that can prevent stress and increase endurance during a pandemic. Suggestion: It is necessary to conduct training and assistance in hygiene food processing to produce processed innovations that can be used to increase body immunity.

This study aims to determine changes in the morphology, the number, and diameter of Langerhans Islets cells in White Sprague Dawley rats with diabetes mellitus after treatment of ethyl acetate fraction synom lime and honey mixture. This study is an experimental study which divided into 5 groups, the control group (negative and positive control), the treatment group induced by alloxan and were then given the ethyl acetate fraction of mixed sinom, lime, and honey with a dose of 50; 100; 150; and 200 mg/kg. The histopathological features were observed by HE staining. The results of statistical tests on the average number of cells at a dose of 150 mg/kg BW showed a significant difference (p0.05) from other doses, as well as the observation of the morphology of the islets of Langerhans cells showed the best regeneration of islets of Langerhans in the treatment group with the ethyl acetate fraction of mixed synom drink. Lime and honey are characterized by an increase in the size of the islets of Langerhans, a uniform endocrine cell shape and an almost normal spherical cell nucleus. Penelitian ini bertujuan untuk mengetahui perubahan morfologi sel pulau Langerhans, jumlah, dan diameter pulau Langerhans dari tikus putih Sprague Dawley diabetes melitus pasca terapi fraksi etil asetat sinom campuran jeruk nipis dan madu. Penelitian ini merupakan penelitian eksperimental yang terbagi dalam 5 kelompok, kelompok kontrol (kontrol negatif dan kontrol positif), kelompok perlakuan yang telah diinduksi alloxan kemudian diberikan hasil fraksi etil asetat minuman sinom campuran jeruk nipis dan madu dengan dosis 50; 100; 150; dan 200 mg/kgBB. Gambaran histopatologi diamati dengan pewarnaan HE. Hasil uji statistik rerata jumlah sel dosis 150 mg/kgBB menunjukkan perbedaan nyata (p0,05) dari dosis lainya, demikian juga terhadap pengamatan morfologi sel pulau Langerhans menunjukkan adanya regenerasi sel pulau Langerhans paling baik pada kelompok perlakuan hasil fraksi etil asetat minuman sinom campuran jeruk nipis dan madu ditandai dengan peningkatan ukuran pulau Langerhans, bentuk sel endokrin yang seragam serta intil sel berbentuk bulat hampir mendekati normal.

ABSTRAK                                       Latar Belakang: ibu yang melahirkan dengan persalinan Sectio Caesarea (SC) terbukti secara bermakna meningkatkan kematian ibu. Risiko banyak terjadi pada persalinan SC yang direncanakan elektif(Villar, 2006). Beberapa tahun terakhir angka kejadian tindakan persalinan melalui SC meningkat melebihi target WHO. Upaya Vaginl Birth After Caesarean (VBAC) masih sedikit peminatnya (ACOG, 2010). Ibu yang takut menjalani proses persalinan secara normal disebabkan oleh banyak faktor salah satunya adalah masalah kecemasan dan ketidaktahuan mereka. Tujuan penelitian ini adalah untuk mengetahi apakah pengetahuan ibu hamil berhubungan dengan keinginan ibu untuk memilih metode VBAC.Metode: metode yang digunakan dalam penelitian ini adalah analitik observasional dengan Rancangan Cross Sectional Study. Sampel dalam penelitian ini adalah ibu dengan riwayat SC non indikasi mutlak sebanyak 1 kali. Pada penelitian ini instrumen yang akan digunakan adalah lembar kuesioner. Analisis data menggunakan Chi Square.Hasil: hasil penelitian menunjukan bahwa terdapat hubungan antara pengetahuan ibu dengan keinginan ibu dalam memilih metode persalinan VBAC dengan nilai p= 0.01.Kesimpulan: dapat disimpulkan bahwa pengetahuan berhubungan dengan keinginan ibu dalam memilih metode persalinan VBAC sehingga disarankan bagi penyedia layanan kesehatan dapat membantu ibu dan memotivasi ibu dengan memberikan kelas ibu hamil sebelum ibu melalui proses persalinan.Kata kunci: pengetahuan, ibu hamil, VBACABSTRACTBackground: mother who was giving the birth a child with Sectio Caesarea (SC) is proved significant to improve mother’s death. Many risks occur to childbirth SC that has been planned effectively (Villar, 2006). In recent years the case of childbirth through the SC has increased beyond WHO targets. The efforts to Vaginl Birth After Caesarean (VBAC) are still relatively small in demand (ACOG, 2010). Mothers who are afraid to process childbirth normally are caused by many factors, one of which is their anxiety and ignorance. The aim of this study was to determine whether the knowledge of pregnant women is related to the motivation of mothers to choose the VBAC method.Methods: the method used in this research is observational analytic with Cross Sectional Study Design. The sample in this study was mothers with a history of non-absolute indication as much as 1 time. In this study the instrument to be used was a questionnaire sheet. Data analysis is used Chie Square.Results: the results showed that there was a relationship between mother's knowledge and mother's in choosing the VBAC delivery method with p = 0.01.Conclusion: It can be concluded that knowledge is related to the motivation of mothers by selected the VBAC childbirth method so that it is recommended for health care providers to help mothers and motivate mothers by giving grades of pregnant women before mothers to process through childbirth.Keywords: knowledge, pregnant women, VBAC

ABSTRAK<br />Antibody poliklonal sebagai sumber dasar skrining antigen yang dihasilkan melalui teknik kloning gen penyandi protein escretory surface antigen (ESA) Toxoplasma gondii. Skrining diperlukan untuk mengetahui reaksi imunitas protein rekombinan ESA yang mampu mengenali protein anti-ESA secara biomolekuler dan spesifik. Penelitian ini bertujuan untuk menghasilkan antibodi poliklonal untuk digunakan dalam pengembangan teknik analisa Western-blot. Antigen yang digunakan berasal dari crude protein ESA, dari stadium takizoit. Mencit BALB/c betina berumur 8-10 minggu disuntik crude protein ESA pada rongga peritoneum mencit. Penyuntikan ulang (booster) diulang sebanyak 5 kali dengan interval waktu 14 hari. Serum dari mencit dikoleksi dan diukur titernya dengan ELISA, dan dilanjutkan pada analisa Western-blot. Hasil analisis Western-blot menunjukkan bahwa antibodi poliklonal yang dikembangkan pada mencit mampu bereaksi secara spesifik terhadap antigen yang dihasilkan melalui rekayasa genetik. Berdasarkan hal ini dapat disimpulkan bahwa antibodi poliklonal yang dikembangkan pada mencit telah berhasil dan dapat digunakan sebagai reagen immunoproteomik.<br />Kata Kunci : antibody poliklonal, analisa Western-blot, Toxoplasma gondii.<br />ABSTRACT<br />Polyclonal antibody is a basic resource in screening antigen from recombinant DNA technology of excretory surface antigen of Toxoplasma gondii. Screening is using to analyze the immunity reaction of recombinant protein to recognize the antibody in specific and molecular. The aim of this research is to produce excretory surface antigen (ESA) of Toxoplasmaa gondii and can developed for Western-blot analyze. The antigen is from cruse protein isolated tachyzote of Toxoplasma gondii. Female mice are in age 8-10 weeks, injected in the peritoneum. The booster is injected for five times, in interval every 14 days. The serums are collected and analyze by ELISA and use it on Western-blot. The result is the polyclonal antibody showed reaction with the antigen from genetic engineering. From this result it can concluded that the developing of this antibody is successful and it can be used as an immunoproteomic reagent.<br />Keyword : Polyclonal antibody, Western-blot, Toxoplasma gondi