Activation of macrophages stimulates the release of various inflammatory mediators which become targets for the development of anti-inflammatory drugs. The combination of Curcuma xanthorrhiza and Physalis angulata extracts was investigated for its anti-inflammatory properties and synergistic effects. Separate extraction with ethanol was applied for both materials, followed by the determination of the characteristics of each extract. To assess the production of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6), each extract and its combination was tested on lipopolysaccharide-(LPS)-induced RAW 264.7 cells using the enzyme-linked immunosorbent assay method. Nitric oxide production was determined by measuring the nitrite content using the Griess method. The cell viability was determined using the MTT method. Both extracts were also able to inhibit inflammatory mediators such as TNF-α, IL-6, and NO. The combination of the two extracts was dominated by a synergistic effect (C < 1) in all concentration ratios, but increasing the concentration ratio gave a cytotoxic effect. It can be concluded that both extracts possessed an anti-inflammatory activity on the LPS-induced RAW 264.7 cells model, and the combination of these extracts displayed synergistic anti-inflammatory activity.
Analgetik adalah zat atau suatu bahan yang dapat menekan ataupun menghilangkan rasa nyeri dengan tidak menghilangkan kesadaran. Daun belimbing wuluh mengandung flavonoid yang memiliki potensi sebagai analgetik. Tujuan dari penelitian ini untuk mengetahui apakah ekstrak daun dari tanaman Averrhoa bilimbi L. memiliki aktivitas analgetik terhadap mencit putih jantan. Penelitian ini menggunakan rancangan penelitian eksperimental murni dengan tipe penelitian adalah post test only control group design Pengujian aktivitas analgetik ditunjukan dengan adanya respon geliat kemudian dapat dilakukan uji analisis statistika One Way ANOVA dan Post Hoc LSD. Hasil penelitian menunjukan bahwa ekstrak etanol 96% daun belimbing wuluh memiliki aktivitas analgetik pada mencit putih jantan. Berdasarkan uji One Way Anova, antara kelompok perlakuan dengan kontrol positif memiliki perbedaan bermakna dengan nilai signifikansi sebesar 0.000 (p<0.05). Hasil analisis data uji post hoc LSD menunjukkan bahwa terdapat perbedaan bermakna antara kelompok perlakuan dosis 125 mg/ kg BB, 250 mg/ kg BB dengan kontol positif. Simpulan dari penelitian ini adalah dosis 500 mg/ kg BB ekstrak etanol 96% daun belimbing wuluh (Averrho bilimbi L) pada mencit memiliki efek analgesik dengan persentase penghambat geliat yaitu 68.49% dan mendekati kontrol positif parasetamol dengan dosis 65mg/ kg BB sebesar 80.36%.
Background: Acnes vulgaris is a skin disease that can reduce self-confidence in adolescence and adulthood. This disease can be caused by infection with Propionbacterium acnes bacteria and treatment of this disease using antibiotics causes a lot of resistances. One alternative that can be developed to overcome this problem is the use of herbal medicines. Objective: To determine the antibacterial activity of 96% ethanol extract sambiloto leaf (Androgaphis paniculata (Burm.f.) Nees) in inhibiting the growth of Propionbacterium acnes bacteria. Method: The maceration method was used in the preparation of extracts and a qualitative test of active compounds was carried out. The antibacterial activity test was carried out using the disc diffusion method. Results: Qualitatively identified in the extract contains several active compounds like alkaloids, flavonoids, tannins, triterpenoids and saponins. 96% ethanol extract of sambiloto leaf with concentrations of 25, 50, 75, and 100% w/v had antibacterial activity against Propionibacterium acnes, indicated by the diameter of the inhibition zone at each concentration. Conclusion: 96% ethanol extract of sambiloto leaf (Andrographis panuculata (Burm.f.) Nees) has antibacterial activity against the growth of Propionibacterium acnes bacteria where the category of strong antibacterial activity was found in samples with concentrations of 75 and 100% w/v with the diameter of the inhibition zone formed respectively 11.26±0.44 mm and 16.52 ± 1.33 mm. Keywords: Andrographis paniculata (Bum.f.) Nees, antibacterial, disc diffusion, 96% ethanol extract, Propionibacterium acnes
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