Background: Infertility is a problem in need for solution. Seminal fluid analysis is the first test that needs to be done to study the shape, count, and activity of sperm. Sperm have to move and reach the ovum to initiate fertilization. Many researchers and fertility doctors work hard for this purpose. The first studies clearly indicate that human sperm motility as well as velocity can be improved by laser irradiation Objective: The aim of our study was to combat infertility by enhancing sperm motility, and also to evaluate the role of laser activity in solving infertility problems. Materials and Methods: 50 semen samples were selected from infertile patients referred to the" High Institute for Infertility Diagnosis and ART, Al-Nahrain University", through the time span from December 2021 to April 2022. Standard seminal analysis was done to measure sperm concentration, motility, morphology, and agglutination according to WHO 2010 guidelines. Results: the present work found that the red laser exposure to the samples of asthenozoospermia for 10, 20, and 30 min resulted in a significant increase in the percentage of progressive sperm motility compared to before exposure. However, there was no significant difference in the morphologically normal sperm percentage compared to before the laser exposure. Conclusion: Sperm motility increased and motility quality showed an improvement in the treated samples, due to low level laser.