Distribution and plant uptake of soil Cd as influenced by organic matter and soil type were investigated in a greenhouse experiment. Three soils (a sand, sandy loam and clay loam) were used. The rates of organic matter in its moist state added were 0,20,40, 80, 160 and 320 g kg-.' of the air-dried soil on mass basis. Ryegrass (Lolium mult$orum L.) was used as a test crop. Soil Cd was analysed by a sequential extraction technique and by extraction with I M NH,NO, and 0.005 M DTPA. The exchangeable fraction of Cd as determined by 1 M MgCI, in the sequential extraction procedure increased, whereas the Fe-Mn oxidebound fraction decreased, with increasing levels of organic matter addition in all three soils. The dry matter yields of ryegrass were not affected by the addition of organic matter, but the Cd concentrations in both cuts of ryegrass decreased with increasing amounts of organic matter added. The plant Cd was highly but negatively correlated to soil CEC. At any level of organic matter addition, the decrease in Cd concentration of ryegrass was in the order: sand > sandy loam >clay loam.
The substrate of mushroom can be polluted with heavy metals and subsequently contaminate mushroom, which requires alternative solutions to reduce associated environmental and human health risks. The effects of amendment application on alleviating Cu and Cd toxicities to Pleurotus cornucopiae were investigated in a cultivated bag experiment conducted with the naturally contaminated substrate. Addition of combined amendments (sodium bentonite, silicon fertilizer, activated carbon, and potassium dihydrogen phosphate) increased the P. cornucopiae biomass and substrate pH. Cu and Cd concentration in P. cornucopiae as well as the available Cu and Cd in substrate reduced for the presence of amendments, and the silicon fertilizer had the biggest inhibition on metal uptake. The smallest amount of Cu and Cd in P. cornucopiae was only 30.8 and 5.51% of control, respectively. Moreover, application of amendments also decreased malondialdehyde (MDA) and hydrogen peroxide (H2O2) level in metal-stressed mushroom by 4.38-53.74 and 8.90-58.42% relative to control, respectively. The decreased oxidative stress could well contribute to the growth of P. cornucopiae, and the elevated substrate pH might lead to the lower metal availability, thus resulting in the reduction of metal accumulation in mushroom. These above results suggest that application of combined amendments in mushroom substrate could be implemented in a general scheme aiming at controlling metal content in P. cornucopiae.
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