Gene therapy is one of the most attractive fields in tumor therapy. In past decades, significant progress has been achieved. Various approaches, such as viral and non-viral vectors and physical methods, have been developed to make gene delivery safer and more efficient. Several therapeutic strategies have evolved, including gene-based (tumor suppressor genes, suicide genes, antiangiogenic genes, cytokine and oxidative stress-based genes) and RNA-based (antisense oligonucleotides and RNA interference) approaches. In addition, immune response-based strategies (dendritic cell– and T cell–based therapy) are also under investigation in tumor gene therapy. This review highlights the progress and recent developments in gene delivery systems, therapeutic strategies, and possible clinical directions for gene therapy.

In order to explore the disintegration characteristics of compacted loess, through the unsaturated soil disintegration instrument by independently developed,conduct the soaking disintegration experimental study on remolded soil unsaturated samples,obtained a series of experimental results:①The curves can be divided into the slow disintegration, rapid disintegration and stabilization of disintegration in three stages，the degree of compaction and water rate impact on rapidly disintegrating stage;②When the degree of compaction is certain,the disintegration rate decreased with the water contented increasing,the decreased amplitude is increasing with the moisture content increased to18%,the disintegration characteristics disappeared with the moisture content increased to 22%;③When the initial moisture content is certain, the disintegration rate decreased with the degree of compaction increasing, The time significantly prolonged when the disintegration completed. the decreased amplitude is increasing with the degree of compaction increased to 95%,the disintegration characteristics basically disappeared. Based on the relationship of degree of compaction and the moisture content to void ratio, the thesis establishes the model between the disintegration rate and the effective porosity ratio,the formula can be used to calculate the disintegration rate of compacted loess.The specific conclusions are:①When the critical moisture content increased to 22%,the disintegration characteristics disappeared;②When the critical degree of compaction increased to95%，the disintegration characteristics also disappeared.

Based on the understanding of diseases of building’s foundation and manmade slope caused by water capillary rise, this thesis carried out a large number of laboratory tests by selfdeveloped absorption permeameter for unsaturated soil, in order to explore the capillary transport law of unsaturated soil. The thesis obtains some valuable results and conclusions: The moisture content shows minishing trend as the seepage fronts increasing, and reflects the gravitational potential of normal relations with the capillary action; (2) the water seepage frontal Hω% relation curve has no obvious inflection point, but the initial water content has an certain influence on the capillary moisture content distribution, the moisture content uses the initial moisture content of 12.5%, 9.8%, 8.0% and5.3% for testing showed an increasing trend at 4 cm and 16 cm sections, and the moisture content at the same section increased with the initial moisture content increasing, this reflects the rule that the smaller the moisture content is, the greater the matric potential, the water absorption capacity becomes stronger. (3)The moisture content difference between 4 cm and 16 cm of the initial moisture content is 12.5%, 9.8%, 8.0% and 9.8% is 1.48 %, 1.5 %, 1.7 % and 2.2 %, indicating that the smaller the initial moisture content is, the difference at the vertical section is greater. Finally, based on the relationship of Hω% curve, the thesis establishes the grey correlation prediction model, and forecasts the maximum capillary height of Lishi loess is 182cm.

A high accurate adaptive temperature control algorithm is proposed and implemented for a thermostatic chemical detecting device. The proposed algorithm combines the advantages of PID control and Fuzzy control which are high accuracy and easy control. The algorithm flow chart and fuzzy reasoning rules are presented. The experiment results and actual operation show that the algorithm has much better performance on adaptability, stability, accuracy than simple PID control and has a great application prospect on the field of constant-temperature chemical apparatus.

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